Influenţa unor compuşi biologic activi noi asupra principalilor indici ai metabolismului glucidic în hepatopatia experimentală ; Influence of some new biologically active compounds on the main indicators of carbohydrate metabolism in experimental hepatopathy

Laboratorul ştiinţific Biochimie, USMF „Nicolae Testemiţanu” ; The experimental toxic hepatopathy (HP) was modeled by long term administration of CCl4. It was established that CCl4 caused pronounced disorders of carbohydrate metabolism manifested by the increased level of the metabolites – lactate, pyruvate and malate, and changes in tissue enzyme activity – the increased activity of lactate dehydrogenase (LDH) and the reduction of the functionality of the main dehydrogenases of the Krebs cycle – NADP-dependent malate dehydrogenase (NADP-MDH), NADP-dependent isocitrate dehydrogenase (ICDHNADP) and succinate dehydrogenase (SDH). The administration of local biologically active compounds (BAC) – CMD-8, CMJ-23 and CMJ-33, of Spirulina Platensis sulphatated polysaccharides (SSP) and of the remedy BioR Fe-Se in the toxic HP exerted important influences both on the evolution of metabolites content changes and on the activity of the investigated enzymes. The tested BAC succeeded to reduce the intensity of the toxic effects of CCl4 on hepatocytes by inducing a tendency of normalization and even by restoring to normal levels of the carbohydrate metabolites content, as well as of the functionality of studied cellular enzymes. Pe animale de laborator s-a modelat hepatopatia (HP) toxică prin administrarea de durată a CCl4. S-a stabilit că CCl4 a provocat tulburări pronunţate ale metabolismulu glucidic care s-au manifestat prin creşterea nivelului de metaboliţi – lactat, piruvat şi malat şi modificări ale activităţii enzimelor tisulare – creşterea activităţii lactatdehidrogenazei (LDH) şi reducerea funcţionalităţii principalelor dehidrogenaze ale ciclului Krebs – malatdehidrogenazei NADPdependente (MDH-NADPd), izocitrat dehidrogenazei NADP-dependente (ICDH-NADPd) şi succinatdehidrogenazei (SDH). Administrarea compuşilor biologic activi (CBA) autohtoni – CMD-8, CMJ-23 şi CMJ-33, polizaharidelor sulfatate din spirulină şi a remediului BioR Fe-Se în HP toxică a exercitat influenţe importante atât asupra modificărilor ...