Molecular recognition of Escherichia coli R1-type core lipooligosaccharide by DC-SIGN ; Molecular recognition of Escherichia coli R1-type core lipooligosaccharide by DC-SIGN.
In: EISSN: 2589-0042 ; iScience ; https://hal.science/hal-04467850 ; iScience, 2024, 27 (2), pp.108792. ⟨10.1016/j.isci.2024.108792⟩, 2024
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academicJournal
Zugriff:
International audience ; Due to their ability to recognize carbohydrate structures, lectins emerged as potential receptors for bacterial lipopolysaccharides (LPS). Despite growing interest in investigating the association between host receptor lectins and exogenous glycan ligands, the molecular mechanisms underlying bacterial recognition by human lectins are still not fully understood. We contributed to fill this gap by unveiling the molecular basis of the interaction between the lipooligosaccharide of Escherichia coli and the dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin (DC-SIGN). Specifically, a combination of different techniques, including fluorescence microscopy, surface plasmon resonance, NMR spectroscopy, and computational studies, demonstrated that DC-SIGN binds to the purified deacylated R1 lipooligosaccharide mainly through the recognition of its outer core pentasaccharide, which acts as a crosslinker between two different tetrameric units of DC-SIGN. Our results contribute to a better understanding of DC-SIGN-LPS interaction and may support the development of pharmacological and immunostimulatory strategies for bacterial infections, prevention, and therapy.
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Molecular recognition of Escherichia coli R1-type core lipooligosaccharide by DC-SIGN ; Molecular recognition of Escherichia coli R1-type core lipooligosaccharide by DC-SIGN.
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Autor/in / Beteiligte Person: | Nieto-Fabregat, Ferran ; Marseglia, Angela ; Thépaut, Michel ; Kleman, Jean-Philippe ; Abbas, Massilia ; Le Roy, Aline ; Ebel, Christine ; Maalej, Meriem ; Simorre, Jean-Pierre ; Laguri, Cedric ; Molinaro, Antonio ; Silipo, Alba ; Fieschi, Franck ; Marchetti, Roberta ; Dipartimento di Scienze Chimiche ; University of Naples Federico II = Università degli studi di Napoli Federico, II ; Institut de biologie structurale (IBS - UMR 5075) ; Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA) |
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Zeitschrift: | EISSN: 2589-0042 ; iScience ; https://hal.science/hal-04467850 ; iScience, 2024, 27 (2), pp.108792. ⟨10.1016/j.isci.2024.108792⟩, 2024 |
Veröffentlichung: | HAL CCSD ; Elsevier, 2024 |
Medientyp: | academicJournal |
DOI: | 10.1016/j.isci.2024.108792 |
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