Genetic identification of bat species for pathogen surveillance across France
In: ISSN: 1932-6203, 2022
Online
academicJournal
Zugriff:
International audience ; With more than 1400 chiropteran species identified to date, bats comprise one-fifth of all mammalian species worldwide. Many studies have associated viral zoonoses with 45 different species of bats in the EU, which cluster within 5 families of bats. For example, the Serotine bats are infected by European Bat 1 Lyssavirus throughout Europe while Myotis bats are shown infected by coronavirus, herpesvirus and paramyxovirus. Correct host species identification is important to increase our knowledge of the ecology and evolutionary pattern of bat viruses in the EU. Bat species identification is commonly determined using morphological keys. Morphological determination of bat species from bat carcasses can be limited in some cases, due to the state of decomposition or nearly indistinguishable morphological features in juvenile bats and can lead to misidentifications. The overall objective of our study was to identify insectivorous bat species using molecular biology tools with the amplification of the partial cytochrome b gene of mitochondrial DNA. Two types of samples were tested in this study, bat wing punches and bat faeces. A total of 163 bat wing punches representing 22 species, and 31 faecal pellets representing 7 species were included in the study. From the 163 bat wing punches tested, a total of 159 were genetically identified from amplification of the partial cyt b gene. All 31 faecal pellets were genetically identified based on the cyt b gene. A comparison between morphological and genetic determination showed 21 misidentifications from the 163 wing punches, representing ~12.5% of misidentifications of morphological determination compared with the genetic method, across 11 species. In addition, genetic determination allowed the identification of 24 out of 25 morphologically non-determined bat samples. Our findings demonstrate the importance of a genetic approach as an efficient and reliable method to identify bat species precisely.
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Genetic identification of bat species for pathogen surveillance across France
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| Autor/in / Beteiligte Person: | Arnaout, Youssef ; Djelouadji, Zouheira ; Robardet, Emmanuelle ; Cappelle, Julien ; Cliquet, Florence ; Touzalin, Frédéric ; Jimenez, Giacomo ; Hurstel, Suzel ; Borel, Christophe ; Picard-Meyer, Evelyne ; Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN) ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) ; VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) ; Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP) ; VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) ; Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques (UMR EPIA) ; School of Biology and Environmental Sciences (SBES) ; University College Dublin Dublin (UCD) ; Commission de Protection des Eaux, du Patrimoine, de l'Environnement, du Sous-sol et des Chiroptères de Lorraine (CPEPESC Lorraine) ; LPO Alsace (LPO Alsace) ; Partenaires, IRSTEA ; Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) ; Comission de protection des eaux, du patrimoine, de l'environnement, du sous-sol et des chiroptères (CPEPESC) ; ONG ; This work was supported by the French National Agency for Food, Environmental and Occupational Health & Safety and VetAgro Sup laboratory for Leptospira. |
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| Zeitschrift: | ISSN: 1932-6203, 2022 |
| Veröffentlichung: | HAL CCSD ; Public Library of Science, 2022 |
| Medientyp: | academicJournal |
| ISBN: | 978-0-00-834805-2 (print) ; 0-00-834805-7 (print) |
| DOI: | 10.1371/journal.pone.0261344 |
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