Figure 3. tim mRNA cycling in sLNvs and LNds shows similar trends as protein cycling as observed by FISH. ; (A) Representative brain showing tim (left panel), pdf (middle panel) and a composite of both channel (right panel). Tim probes label all clock neurons and glial cells, whereas the pdf probes only label sLNvs and lLNvs. (B–C) tim mRNA cycling in LD 12:12 in sLNvs (B) and LNds (C). Control flies (black data points ± SEM, pooled GAL4 and UAS, n = 10) show high amplitude cycling with peak levels at the beginning of the night. Silencing the clock network (red data points ± SEM, n = 5) has only little effect on cycling amplitude or timing in LD. (D–E) tim mRNA cycling in DD5 in sLNvs (D) and LNds (E). Control flies (black data points ± SEM, pooled GAL4 and UAS, n = 10) show high amplitude cycling with peak levels at the beginning of the night. Silencing the sLNvs (D) leads to an overall reduction of tim mRNA levels and a loss of rhythmicity. In the LNds (E) silencing did not decrease cycling amplitude or shifted peak mRNA expression.

Figure 3. tim mRNA cycling in sLNvs and LNds shows similar trends as protein cycling as observed by FISH. ; (A) Representative brain showing tim (left panel), pdf (middle panel) and a composite of both channel (right panel). Tim probes label all clock neurons and glial cells, whereas the pdf probes only label sLNvs and lLNvs. (B–C) tim mRNA cycling in LD 12:12 in sLNvs (B) and LNds (C). Control flies (black data points ± SEM, pooled GAL4 and UAS, n = 10) show high amplitude cycling with peak levels at the beginning of the night. Silencing the clock network (red data points ± SEM, n = 5) has only little effect on cycling amplitude or timing in LD. (D–E) tim mRNA cycling in DD5 in sLNvs (D) and LNds (E). Control flies (black data points ± SEM, pooled GAL4 and UAS, n = 10) show high amplitude cycling with peak levels at the beginning of the night. Silencing the sLNvs (D) leads to an overall reduction of tim mRNA levels and a loss of rhythmicity. In the LNds (E) silencing did not decrease cycling amplitude or shifted peak mRNA expression.