Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers
In: ISSN: 1874-3919, 2022
Online
academicJournal
Zugriff:
International audience ; The avian egg perivitelline layer (PL) is a proteinaceous structure that encloses the egg yolk. It consists of the inner and the outer perivitelline layers (IPL and OPL, respectively) that are assumed to play distinct roles in bird reproduction. To gain insight into their respective function, we analyzed the proteome of IPL and OPL in chicken unfertilized eggs after mechanical separation, using a GeLC-MS/MS strategy. Of the 412 proteins identified, 173 proteins were uniquely recovered in IPL and 98 proteins in OPL, while 141 proteins were identified in both sublayers. Genes coding the most abundant proteins were shown to be expressed either in the liver/ovary (IPL formation) or in the oviduct (OPL formation), but rarely in both. The presence of oviduct-specific proteins (including LYZ, VMO1, AvBD11, PTN, OVAL and LOC10175704) in IPL strongly suggests that they participate in the physical association of IPL to OPL, whose tight attachment was further evidenced by analyses of IPL/OPL interfaces (by scanning electron microscopy). Functional annotation of identified proteins revealed functions associated with fertilization and early development for IPL, while OPL would rather participate in egg defense and embryogenesis. Collectively, our data highlight the complementary functions of IPL and OPL that are major determinants of bird reproductive success.
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Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers
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Autor/in / Beteiligte Person: | Brégeon, Mégane ; Tomas, Daniel ; Bernay, Benoît ; Zatylny-Gaudin, Céline ; Georgeault, Sonia ; Labas, Valérie ; Réhault-Godbert, Sophie ; Guyot, Nicolas ; Biologie des Oiseaux et Aviculture (BOA) ; Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Physiologie de la reproduction et des comportements Nouzilly (PRC) ; Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Plate-forme Phénotypage par imagerie in/ex vivo de l'Animal à la Molécule (Plate-forme PIXANIM) ; Interactions Cellules Organismes Environnement (ICORE) ; Université de Caen Normandie (UNICAEN) ; Normandie Université (NU)-Normandie Université (NU)-CHU Caen ; Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) ; Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) ; Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) ; Plateforme des Microscopies ; Université de Tours (UT) ; This work was supported by the Région Centre-Val de Loire grant number 2017-119983 and the Agence Nationale de la Recherche grant number ANR-19-CE21-0006 . ; ANR-19-CE21-0006,EQLIPSE,Amélioration de la qualité et des défenses antimicrobiennes internes de l'oeuf(2019) |
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Zeitschrift: | ISSN: 1874-3919, 2022 |
Veröffentlichung: | HAL CCSD ; Elsevier, 2022 |
Medientyp: | academicJournal |
DOI: | 10.1016/j.jprot.2022.104489 |
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