Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup
In: International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12746, 2021
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Zugriff:
The O-antigen is the outermost component of the lipopolysaccharide layer in Gram-negative bacteria, and the variation of O-antigen structure provides the basis for bacterial serological diversity. Here, we determined the O-antigen structure of an Escherichia coli strain, LL004, which is totally different from all of the E. coli serogroups. The tetrasaccharide repeating unit was determined as →4)-β-d-Galp-(1→3)-β-d-GlcpNAc6OAc(~70%)-(1→3)-β-d-GalpA-(1→3)-β-d-GalpNAc-(1→ with monosaccharide analysis and NMR spectra. We also characterized the O-antigen gene cluster of LL004, and sequence analysis showed that it correlated well with the O-antigen structure. Deletion and complementation testing further confirmed its role in O-antigen biosynthesis, and indicated that the O-antigen of LL004 is assembled via the Wzx/Wzy dependent pathway. Our findings, in combination, suggest that LL004 should represent a novel serogroup of E. coli.
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Structural Determination and Genetic Identification of the O-Antigen from an Escherichia coli Strain, LL004, Representing a Novel Serogroup
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Autor/in / Beteiligte Person: | Wang, Jing ; Xu, Yujuan ; Qin, Chunjun ; Hu, Jing ; Yin, Jian ; Guo, Xi |
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Zeitschrift: | International Journal of Molecular Sciences; Volume 22; Issue 23; Pages: 12746, 2021 |
Veröffentlichung: | Multidisciplinary Digital Publishing Institute, 2021 |
Medientyp: | academicJournal |
DOI: | 10.3390/ijms222312746 |
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