FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer ...
figshare, 2024
academicJournal
Zugriff:
Background The development of radioresistance seriously hinders the efficacy of radiotherapy in lung cancer. However, the underlying mechanisms by which radioresistance occurs are still incompletely understood. The N6-Methyladenosine (m6A) modification of RNA is involved in cancer progression, but its role in lung cancer radioresistance remains elusive. This study aimed to identify m6A regulators involved in lung cancer radiosensitivity and further explore the underlying mechanisms to identify therapeutic targets to overcome lung cancer radioresistance. Methods Bioinformatic mining was used to identify the m6A regulator IGF2BP2 involved in lung cancer radiosensitivity. Transcriptome sequencing was used to explore the downstream factors. Clonogenic survival assays, neutral comet assays, Rad51 foci formation assays, and Annexin V/propidium iodide assays were used to determine the significance of FBW7/IGF2BP2/SLC7A5 axis in lung cancer radioresistance. Chromatin immunoprecipitation (ChIP)-qPCR ...
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FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer ...
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Autor/in / Beteiligte Person: | Zhou, Zhiyuan ; Zhang, Bin ; Deng, Yue ; Deng, Suke ; Li, Jie ; Wei, Wenwen ; Wang, Yijun ; Wang, Jiacheng ; Feng, Zishan ; Che, Mengjie ; Yang, Xiao ; Meng, Jingshu ; Li, Yan ; Hu, Yan ; Sun, Yajie ; Wen, Lu ; Huang, Fang ; Sheng, Yuhan ; Wan, Chao ; Yang, Kunyu |
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Veröffentlichung: | figshare, 2024 |
Medientyp: | academicJournal |
DOI: | 10.6084/m9.figshare.c.7047301.v1 |
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