QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
Springer-Verlag, 2021
Online
academicJournal
Zugriff:
The two most commonly used wine microorganisms, Saccharomyces cerevisiae yeast and Oenococcus oeni bacteria, are responsible for completion of alcoholic and malolactic fermentation (MLF), respectively. For successful co-inoculation, S. cerevisiae and O. oeni must be able to complete fermentation; however, this relies on compatibility between yeast and bacterial strains. For the first time, quantitative trait loci (QTL) analysis was used to elucidate whether S. cerevisiae genetic makeup can play a role in the ability of O. oeni to complete MLF. Assessment of 67 progeny from a hybrid S. cerevisiae strain (SBxGN), co-inoculated with a single O. oeni strain, SB3, revealed a major QTL linked to MLF completion by O. oeni. This QTL encompassed a well-known translocation, XV-t-XVI, that results in increased SSU1 expression and is functionally linked with numerous phenotypes including lag phase duration and sulphite export and production. A reciprocal hemizygosity assay was performed to elucidate the effect of the gene SSU1 in the SBxGN background. Our results revealed a strong effect of SSU1 haploinsufficiency on O. oeni’s ability to complete malolactic fermentation during co-inoculation and pave the way for the implementation of QTL mapping projects for deciphering the genetic bases of microbial interactions. ; Louise Bartle, Emilien Peltier, Joanna F. Sundstrom, Krista Sumby, James G. Mitchell, Vladimir Jiranek, Philippe Marullo
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QTL mapping: an innovative method for investigating the genetic determinism of yeast-bacteria interactions in wine
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Autor/in / Beteiligte Person: | Bartle, L. ; Peltier, E. ; Sundstrom, J.F. ; Sumby, K. ; Mitchell, J.G. ; Jiranek, V. ; Marullo, P. |
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Veröffentlichung: | Springer-Verlag, 2021 |
Medientyp: | academicJournal |
ISSN: | 0175-7598 (print) ; 1432-0614 (print) |
DOI: | 10.1007/s00253-021-11376-x |
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