Bacterial metabolism of bile acids promotes peripheral Treg cell generation
In: Nature, 2020
academicJournal
Zugriff:
Intestinal health relies on the immunosuppressive activity of CD4(+) regulatory T (Treg) cells(1). Foxp3 expression defines this lineage and can be induced extrathymically by dietary or commensal-derived antigens in a process assisted by the Foxp3 enhancer CNS1 (conserved non-coding sequence 1)(2–4). Microbial fermentation products including butyrate facilitate the generation of peripherally-induced Treg (pTreg) cells(5–7), indicating that metabolites shape colonic immune cell composition. In addition to dietary components, bacteria modify host-derived molecules, generating a number of bioactive substances. This is epitomized by transformation of bile acids (BAs), which creates a complex pool of steroids(8) presenting a range of physiological functions(9). Here, we screened the major species of deconjugated BAs for their ability to potentiate pTreg cell differentiation. We found that the secondary BA 3β-hydroxydeoxycholic acid (isoDCA) increased Foxp3 induction by acting on dendritic cells (DCs) to diminish their immunostimulatory properties. Farnesoid X receptor ablation in DCs enhanced Treg cell generation and imposed a transcriptional profile similar to isoDCA, suggesting interaction between this BA/nuclear receptor pair. To investigate isoDCA in vivo, we took a synthetic biology approach and designed minimal microbial consortia containing engineered Bacteroides strains. IsoDCA-producing consortia increased colonic RORγt(+) Treg cells in a CNS1-dependent manner, indicative of enhanced extrathymic differentiation.
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Bacterial metabolism of bile acids promotes peripheral Treg cell generation
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Autor/in / Beteiligte Person: | Campbell, Clarissa ; McKenney, Peter T. ; Konstantinovsky, Daniel ; Isaeva, Olga I. ; Schizas, Michael ; Verter, Jacob ; Mai, Cheryl ; Jin, Wen-Bing ; Guo, Chun-Jun ; Violante, Sara ; Ramos, Ruben J. ; Cross, Justin R. ; Kadaveru, Krishna ; Hambor, John ; Rudensky, Alexander Y. |
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Zeitschrift: | Nature, 2020 |
Veröffentlichung: | 2020 |
Medientyp: | academicJournal |
DOI: | 10.1038/s41586-020-2193-0 |
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