OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM
In: ISSN: 1553-7390, 2012
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Zugriff:
Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both meiosis and mitosis, and a central regulator of their activity is the APC/C (Anaphase Promoting Complex/Cyclosome) that is especially required for exit from mitosis. We have shown previously that OSD1 is involved in entry into both meiosis I and meiosis II in Arabidopsis thaliana; however, the molecular mechanism by which OSD1 controls these transitions has remained unclear. Here we show that OSD1 promotes meiotic progression through APC/C inhibition. Next, we explored the functional relationships between OSD1 and the genes known to control meiotic cell cycle transitions in Arabidopsis. Like osd1, cyca1;2/tam mutation leads to a premature exit from meiosis after the first division, while tdm mutants perform an aberrant third meiotic division after normal meiosis I and II. Remarkably, while tdm is epistatic to tam, osd1 is epistatic to tdm. We further show that the expression of a non-destructible CYCA1;2/TAM provokes, like tdm, the entry into a third meiotic division. Finally, we show that CYCA1;2/TAM forms an active complex with CDKA; 1 that can phosphorylate OSD1 in vitro. We thus propose that a functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor.
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OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM
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Autor/in / Beteiligte Person: | Cromer, Laurence ; Heyman, Jefri ; Touati, Sandra ; Harashima, Hirofumi ; Araou, Emilie ; Girard, Chloé ; Horlow, Christine ; Wassmann, Katja ; Schnittger, Arp ; de Veylder, Lieven ; Mercier, Raphaël ; Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech ; Dept Plant Syst Biol ; Flanders Institute for Biotechnology ; Dept Plant Biotechnol & Bioinformat ; Universiteit Gent = Ghent University (UGENT) ; Laboratoire de Biologie du Développement (LBD) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ; Institut de Biologie Moléculaire des Plantes (IBMP) ; Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) ; Trinat Inst Fuer Pflanzenforsch ; Centre National de la Recherche Scientifique ; European Union Interreg IV project ; European Research Council 210867, 20101109 ; Agency for Innovation by Science and Technology in Flanders |
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Zeitschrift: | ISSN: 1553-7390, 2012 |
Veröffentlichung: | HAL CCSD ; Public Library of Science, 2012 |
Medientyp: | academicJournal |
ISBN: | 978-0-00-306840-5 (print) ; 0-00-306840-4 (print) |
DOI: | 10.1371/journal.pgen.1002865 |
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