Protein gels and corresponding western blots of purified recombinant proteins.
2021
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Zugriff:
(A) Protein gel (left) and corresponding western blot (right) probed for GST showing purified 6 μg GST-CBU1241 and 2 μg GST proteins of approximately expected size, 61.5 kDa and 26 kDa respectively. (B) Protein gel (left) and corresponding western blot (right) probed for 6xHis-tag showing purified 4 μg 6xHis-CBU0823 and 1 μg 6xHis-oMLE, each of the approximately expected sizes of 63.5 kDa and 62.3 kDa respectively, and the lack of detectable 6xHis-tagged product in the mock purification negative control. (C) Western blot probed for 3xFLAG of C . burnetii 0823 ::Tn pFLAG-CBU0823 whole cell lysate showing C . burnetii expressing a protein product of approximately expected size, 64.5 kDa, from the complementation plasmid. The marker shown is from the corresponding stain-free protein gel. (TIF)
Titel: |
Protein gels and corresponding western blots of purified recombinant proteins.
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Autor/in / Beteiligte Person: | Janine Hofmann (11273418) ; Mebratu A. Bitew (11273421) ; Miku Kuba (11273424) ; David P. De Souza (2793088) ; Hayley J. Newton (8692020) ; Fiona M. Sansom (8893082) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0255925.s002 |
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