A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
In: ISSN: 1935-2727, 2022
Online
academicJournal
Zugriff:
International audience ; Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans . At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African countries, South America and Western Pacific regions. Majority of cases are spread across West and Central Africa. The mode of transmission is unclear, hindering the implementation of adequate prevention for the population. Currently, early diagnosis and treatment are crucial to minimizing morbidity, costs and preventing long-term disability. Biological confirmation of clinical diagnosis of Buruli ulcer is essential before starting chemotherapy. Indeed, differential diagnosis are numerous and Buruli ulcer has varying clinical presentations. Up to now, the gold standard biological confirmation is the quantitative PCR, targeting the insertion sequence IS 2404 of M . ulcerans performed on cutaneous samples. Due to the low PCR confirmation rate in endemic African countries (under 30% in 2018) for numerous identified reasons within this article, 11 laboratories decided to combine their efforts to create the network “BU-LABNET” in 2019. The first step of the network was to harmonize the procedures and ship specific reagents to each laboratory. With this system in place, implementation of these procedures for testing and follow-up was easy and the laboratories were able to carry out their first quality control with a very high success rate. It is now time to integrate other neglected tropical diseases to this platform, such as yaws or leprosy.
Titel: |
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The “BU-LABNET”
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Autor/in / Beteiligte Person: | Marion, Estelle ; Hycenth, Numfor ; Vedithi, Sundeep Chaitanya ; Robbe-Saule, Marie ; Donkeng, Valérie ; Ganlonon, Line-Marlène ; Dissou, Affolabi ; Ngazoa, Solange Kakou ; Kabedi, Marie-Jose ; Mabika Mabika, Arsène ; Phillips, Richard ; Frimpong, Michael ; Yeboah-Manu, Dorothy ; Walker, Vera Yatta ; Akinwale, Olaoluwa ; Issaka, Maman ; Bretzel, Gisela ; Asiedu, Kinsgley ; Eyangoh, Sara ; Immunology and New Concepts in ImmunoTherapy (INCIT) ; Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) ; Centre Pasteur du Cameroun ; Réseau International des Instituts Pasteur (RIIP) ; University of Cambridge UK (CAM) ; Centre de Diagnostic et de Traitement de la lèpre et de l’ulcère de Buruli Pobè, Benin (CDTLUB) ; Fondation Raoul Follereau Pobe, Benin ; Laboratoire de Référence des Mycobactéries Cotonou, Benin ; Hôpital Centre LAZARET Cotonou, Bénin ; Institut Pasteur de Côte d'Ivoire ; Institut National de Recherche Biomédicale Kinshasa (INRB) ; Centre International de Recherches Médicales de Franceville (CIRMF) ; Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR) ; Kwame Nkrumah University of Science and Technology (KNUST) ; Noguchi Memorial Institute for Medical Research Accra, Ghana (NMIMR) ; University of Ghana ; National Public Health Institute of Liberia (NPHIL) ; Nigerian Institute of Medical Research Yaba, Lagos, Nigeria (NIMR) ; Institut Nationale d'Hygiène Lomé, Togo (INH) ; Ludwig-Maximilians University Munich (LMU) ; Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO) |
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Zeitschrift: | ISSN: 1935-2727, 2022 |
Veröffentlichung: | HAL CCSD ; Public Library of Science, 2022 |
Medientyp: | academicJournal |
DOI: | 10.1371/journal.pntd.0010908 |
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