Flt3-ligand induces adhesion of haematopoietic progenitor cells via a very late antigen (VLA)-4- and VLA-5-dependent mechanism
In: ISSN: 0007-1048, 2003
academicJournal
Zugriff:
International audience ; The adhesion of haematopoietic progenitor cells (HPC) to the bone marrow microenvironment is a process regulated by cytokines. In this study, we have shown that flt3-ligand (FL), a growth factor that controls early haematopoiesis, regulated the function and expression of the beta-1 integrins, very late antigen (VLA)-4 and VLA-5 on HPC. The modulation of the adhesiveness of HPC by FL was studied by adhesion assays on umbilical vein endothelial cells (HUVEC). Stimulation by FL induced two peaks of increased adhesiveness of HPC. The first peak was at around 30 min and was mechanistically related to an activation of the beta-1 integrins, mainly VLA-4 and VLA-5. The second peak was at around 12 h and was related to increased expression of VLA-4 and VLA-5. The control of HPC adhesiveness by FL is a previously unreported property of FL that may be important for the homing and the retention of flt3-expressing HPC within the bone marrow microenvironment.
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Flt3-ligand induces adhesion of haematopoietic progenitor cells via a very late antigen (VLA)-4- and VLA-5-dependent mechanism
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| Autor/in / Beteiligte Person: | Solanilla, Anne ; Grosset, Christophe, F ; Duchez, Pascale ; Legembre, Patrick ; Pitard, Vincent ; Dupouy, Maryse ; Reiffers, Josy ; Boiron, Jean-Michel ; Coulombel, Laure ; Ripoche, Jean ; Belloc, Francis ; Viallard, Jean-François ; Fibrose hépatique et cancer du foie ; Université Bordeaux Segalen - Bordeaux 2-IFR66-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Biothérapies des maladies génétiques et cancers ; Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Centre de Transfusion Sanguine Aquitaine-Limousin (CTS AQUITAINE-LIMOUSIN) ; Centre de Transfusion Sanguine ; Composantes innées de la réponse immunitaire et différenciation (CIRID) ; Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) ; Transfert de Genes a Visee Therapeutique Dans les Cellules Souches ; Modèles de Cellules Souches Malignes et Thérapeutiques (ONCOSTEM) ; Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Interne, Médecine ; CHU Bordeaux-Hôpital Haut-Lévêque CHU Bordeaux ; Bordeaux, CHU |
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| Zeitschrift: | ISSN: 0007-1048, 2003 |
| Veröffentlichung: | HAL CCSD ; Wiley, 2003 |
| Medientyp: | academicJournal |
| DOI: | 10.1046/j.1365-2141.2003.04155.x |
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