The impact of high temperature on the development and quality of in vitro matured beef cattle oocytes and their potential to subsequent embryonic development
Nexus Academic Publishers, 2021
academicJournal
Zugriff:
The study aimed to evaluate the effect of high temperature on cattle oocytes matured in vitro at 39, 41, 42, 43˚C and to evaluate apoptosis through Caspase-activity and DNA fragmentation on produced embryos. Cumulus oocyte complexes (COC) were matured at 39, 41, 42 and 43˚C. Two best maturation temperatures were selected for maturation throughout embryo production. Post maturation, matured oocytes were exposed to standard embryonic conditions. Embryos were evaluated at 3 stages: ≥ 2-4 cell, ≥ 8cell and blastocyst to determine Caspase-3 activity and DNA fragmentation. Maturation of oocytes at 41 and 39˚C demonstrated no significant difference (p>0.05) however, higher maturation rate compared to 42 and 43˚C. Matured oocytes at 39 and 41˚C showed no difference (p>0.05) in embryo development. Blastocysts at 39˚C maturation had higher (p<0.05) nuclei cells compared to those at 41˚C. Caspase-3 activity was comparable at ≥ 2-4 cell and ≥ 8cell for both 39 and 41˚C maturation temperatures, however, 41˚C showed high DNA fragmentation compared to 39˚C maturation group. Blastocyst from oocytes matured at 41˚C had higher Caspase-3 activity and DNA fragmentation than those at 39˚C. Embryos derived from 41˚C maturation temperature resulted in evidence of apoptosis compared to 39˚C. ; Technology Innovation Agency (TIA) and Southern African Science Service Centre for Climate Change and Adaptive Land Management, Council for Scientific and Industrial Research (SASCAL). ; https://nexusacademicpublishers.com/journal/4 ; am2022 ; Animal and Wildlife Sciences
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The impact of high temperature on the development and quality of in vitro matured beef cattle oocytes and their potential to subsequent embryonic development
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Autor/in / Beteiligte Person: | Marble, Nkadimeng ; Van Marle-Koster, Este ; Kgomotso, Lekola ; Lekola, Kgomotso ; Lehloenya, Khoboso Christina ; Pilane, Cyril Mpho ; Nedambale, Tshimangadzo Lucky |
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Veröffentlichung: | Nexus Academic Publishers, 2021 |
Medientyp: | academicJournal |
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