Development of a new generation of miniemulsion based on cottonseed oil with α-tocopherol and ZnO and evaluation of its adjuvant activity
In: PeerJ ; volume 11, page e14981 ; ISSN 2167-8359, 2023
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Zugriff:
Background Emulsions have been widely used as immunological adjuvants. But the use of materials derived from plants such as cottonseed oil, alpha-tocopherol, or minerals such as zinc, as well as their use at the nanometric scale has been little explored. In this study, we develop a new miniemulsion and evaluated its antioxidant and phagocytic capacity, as well as parameters related to immune response stimulation by cytokine expression and antibodies production in a mice model. Methods Formulated CN (cottonseed oil miniemulsion) and CNZ (cottonseed oil miniemulsion whit zinc oxide nanoparticles) miniemulsions were characterized by scanning electronic microscopy SEM, DLS and FT-IR. In murine macrophages, splenocytes and thymocytes primary cultures safety and cytotoxicity were determined by MTT. In macrophages the antioxidant and phagocytic capacity was evaluated. In BALB/c mice, the stimulation of the immune system was determined by the expression of cytokines and the production of antibodies. Results The CN and CNZ presented stability for 90 days. Immediately after preparation, the CN presented a higher particle size (543.1 nm) than CNZ (320 nm). FT-IR demonstrated the correct nanoparticle synthesis by the absence of sulfate groups. CN and CNZ (1.25 to 10 µL/mL) had no toxic effect on macrophages ( p = 0.108), splenocytes ( p = 0.413), and thymocytes ( p = 0.923). All CN and CNZ doses tested induced nitric oxide and antioxidants production in dose dependent manner when compared with control. CN-ovalbumin and CNZ-ovalbumin treatments in femoral subcutaneous tissue area showed inflammation with higher leukocyte infiltration compared with FCA. The intraperitoneal administration with CN, CNZ, and FCA showed a higher total intraperitoneal cells recruitment (CD14 + ) after 24 h of inoculation than control ( p = 0.0001). CN and CNZ increased the phagocyte capacity with respect to untreated macrophages in the Candida albicans -phagocytosis assay. The evaluation of residual CFU indicated that only CN significantly ...
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Development of a new generation of miniemulsion based on cottonseed oil with α-tocopherol and ZnO and evaluation of its adjuvant activity
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Autor/in / Beteiligte Person: | Sobrevilla-Hernández, Gustavo ; Franco-Molina, Moisés Armides ; Zárate-Triviño, Diana G. ; Kawas, Jorge R. ; Hernández-Martínez, Sara Paola ; García-Coronado, Paola Leonor ; Santana-Krímskaya, Silvia Elena ; Alvizo-Báez, Cynthia Aracely ; Rodríguez-Padilla, Cristina ; Fondo Sectorial de Investigación para la Educación, CONACYT, México ; Laboratorio de Inmunología y Virología ; Facultad de Ciencias Biológicas ; Universidad Autónoma de Nuevo León |
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Zeitschrift: | PeerJ ; volume 11, page e14981 ; ISSN 2167-8359, 2023 |
Veröffentlichung: | PeerJ, 2023 |
Medientyp: | academicJournal |
DOI: | 10.7717/peerj.14981 |
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