Generation and Efficacy of Two Chimeric Viruses Derived from GPE(−) Vaccine Strain as Classical Swine Fever Vaccine Candidates
In: Viruses, 2023
academicJournal
Zugriff:
A previous study proved that vGPE(−) mainly maintains the properties of classical swine fever (CSF) virus, which is comparable to the GPE(−) vaccine seed and is a potentially valuable backbone for developing a CSF marker vaccine. Chimeric viruses were constructed based on an infectious cDNA clone derived from the live attenuated GPE(−) vaccine strain as novel CSF vaccine candidates that potentially meet the concept of differentiating infected from vaccinated animals (DIVA) by substituting the glycoprotein E(rns) of the GPE(−) vaccine strain with the corresponding region of non-CSF pestiviruses, either pronghorn antelope pestivirus (PAPeV) or Phocoena pestivirus (PhoPeV). High viral growth and genetic stability after serial passages of the chimeric viruses, namely vGPE(−)/PAPeV E(rns) and vGPE(−)/PhoPeV E(rns), were confirmed in vitro. In vivo investigation revealed that two chimeric viruses had comparable immunogenicity and safety profiles to the vGPE(−) vaccine strain. Vaccination at a dose of 10(4.0) TCID(50) with either vGPE(−)/PAPeV E(rns) or vGPE(−)/PhoPeV E(rns) conferred complete protection for pigs against the CSF virus challenge in the early stage of immunization. In conclusion, the characteristics of vGPE(−)/PAPeV E(rns) and vGPE(−)/PhoPeV E(rns) affirmed their properties, as the vGPE(−) vaccine strain, positioning them as ideal candidates for future development of a CSF marker vaccine.
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Generation and Efficacy of Two Chimeric Viruses Derived from GPE(−) Vaccine Strain as Classical Swine Fever Vaccine Candidates
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Autor/in / Beteiligte Person: | Huynh, Loc Tan ; Isoda, Norikazu ; Hew, Lim Yik ; Ogino, Saho ; Mimura, Yume ; Kobayashi, Maya ; Kim, Taksoo ; Nishi, Tatsuya ; Fukai, Katsuhiko ; Hiono, Takahiro ; Sakoda, Yoshihiro |
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Zeitschrift: | Viruses, 2023 |
Veröffentlichung: | MDPI, 2023 |
Medientyp: | academicJournal |
DOI: | 10.3390/v15071587 |
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