EGF and VEGF increased Notch activity, which promoted epithelial-to-mesenchymal transition and VM morphogenesis in PDAC cells.
2022
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Zugriff:
The expression of (A) NOTCH1 and Notch target genes (B) HEY1 and (C) NRARP were determined in PANC-1 cells treated with EGF or VEGF. (D) The expression levels of an epithelial gene CDH1 and mesenchymal genes, including CDH2 , VIM , TWIST1 , and SNAI1 were determined in PANC-1 cells treated with EGF or VEGF. Notch signaling was inhibited by gamma-secretase inhibitor DBZ at 1 nM, and (E) TWIST1 expression levels and (F-G) number of cells with filopodia was determined and analyzed in PANC-1. Red arrows, filopodia. Scale bars, 100 μm. Data presented ± S.D. *P value < 0.01, **P value < 0.001 (n = 4–5).
Titel: |
EGF and VEGF increased Notch activity, which promoted epithelial-to-mesenchymal transition and VM morphogenesis in PDAC cells.
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Autor/in / Beteiligte Person: | Nontawat Benjakul (14295081) ; Nattapa Prakobphol (14295084) ; Chayada Tangshewinsirikul (4678393) ; Wirada Dulyaphat (14295087) ; Jisnuson Svasti (2427940) ; Komgrid Charngkaew (4385962) ; Thaned Kangsamaksin (4678396) |
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Veröffentlichung: | 2022 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0279001.g005 |
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