Faecalibacterium duncaniae A2-165 regulates the expression of butyrate synthesis, ferrous iron uptake, and stress-response genes based on acetate consumption
In: ISSN: 2693-5015 ; Research Square - Preprint ; https://hal.science/hal-04443517 ; Research Square - Preprint, 2023, 14 (1), pp. 987. ⟨10.1038/s41598-023-51059-3⟩, 2023
academicJournal
Zugriff:
International audience ; The promising next-generation probiotic Faecalibacterium prausnitzii is one of the most abundant acetate-consuming, butyrate-producing bacteria in the healthy human gut. Yet, little is known about how acetate availability affects this bacterium's gene expression strategies. Here, we investigated the effect of acetate on temporal changes in the transcriptome of F. duncaniae A2-165 cultures using RNA sequencing. We compared gene expression patterns between two growth phases (early stationary vs. late exponential) and two acetate levels (low: 3 mM vs. high: 23 mM). Only in low-acetate conditions, a general stress response was activated. In high-acetate conditions, there was greater expression of genes related to butyrate synthesis and to the importation of B vitamins and iron. Specifically, expression was strongly activated in the case of the feoAABC operon, which encodes a FeoB ferrous iron transporter, but not in the case of the feoAB gene, which encodes a second putative FeoAB transporter. Moreover, excess ferrous iron repressed feoB expression but not feoAB. Lastly, FeoB but not FeoAB peptides from strain A2-165 were found in abundance in a healthy human fecal metaproteome. In conclusion, we characterized two early-stationary transcriptomes based on acetate consumption and this work highlights the regulation of feoB expression in F. duncaniae A2-165.
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Faecalibacterium duncaniae A2-165 regulates the expression of butyrate synthesis, ferrous iron uptake, and stress-response genes based on acetate consumption
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Autor/in / Beteiligte Person: | Verstraeten, Sophie ; Layec, Séverine ; Auger, Sandrine, S. ; Juste, Catherine ; Henry, Céline ; Charif, Sawiya ; Jaszczyszyn, Yan ; Sokol, Harry ; Beney, Laurent ; Langella, Philippe ; Thomas, Muriel ; Huillet, Eugénie ; MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Paris Center for Microbiome Medicine (FHU PaCeMM) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière AP-HP ; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) ; Institut de Biologie Intégrative de la Cellule (I2BC) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) ; Procédés Alimentaires et Microbiologiques Dijon (PAM) ; Université de Bourgogne (UB)-Université Bourgogne Franche-Comté COMUE (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
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Zeitschrift: | ISSN: 2693-5015 ; Research Square - Preprint ; https://hal.science/hal-04443517 ; Research Square - Preprint, 2023, 14 (1), pp. 987. ⟨10.1038/s41598-023-51059-3⟩, 2023 |
Veröffentlichung: | HAL CCSD ; springer, 2023 |
Medientyp: | academicJournal |
DOI: | 10.1038/s41598-023-51059-3 |
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