A Novel Antibacterial Compound Decreases MRSA Biofilm Formation Without the Use of Antibiotics in A Murine Model
In: ISSN: 1554-527X ; Journal of Orthopaedic Research ; https://hal.science/hal-04195338 ; Journal of Orthopaedic Research, 2023, ⟨10.1002/jor.25638⟩, 2023
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Zugriff:
International audience ; Despite significant advancements in material science, surgical site infection (SSI) rates remain high and prevention is key. This study aimed to demonstrate the in-vivo safety and antibacterial efficacy of titanium implants treated with a novel broad-spectrum biocidal compound (DBG21) against methicillin-resistant Staphylococcus aureus (MRSA). Titanium (Ti) discs were covalently bound with DBG21. Untreated Ti discs were used as controls. All discs were implanted either untreated for 44 control mice or DBG21-treated for 44 treated mice. After implantation, 1x107 colony forming units (CFU) of MRSA were injected into the operating site. Mice were sacrificed at day 7 and 14 to determine the number of adherent bacteria (biofilm) on implants and in the peri-implant surrounding tissues. Systemic and local toxicity were assessed. At both 7 and 14 days, DBG21-treated implants yielded a significant decrease in MRSA biofilm (3.6 median log10 CFU (99.97%) reduction (p<0.001) and 1.9 median log10 CFU (98.7%) reduction (p=0.037), respectively) and peri-implant surrounding tissues (2.7 median log10 CFU/g (99.8%) reduction (p<0.001) and 5.6 median log10 CFU/g (99.9997%) reduction (p<0.001), respectively). There were no significant differences between control and treated mice in terms of systemic and local toxicity. DBG-21 demonstrated a significant decrease in the number of biofilm bacteria without associated toxicity in a small animal implant model of SSI. Preventing biofilm formation has been recognized as a key element of preventing implant-related infections.
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A Novel Antibacterial Compound Decreases MRSA Biofilm Formation Without the Use of Antibiotics in A Murine Model
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Autor/in / Beteiligte Person: | Bouloussa, Houssam ; Durand, Zoe ; Gibon, Emmanuel ; Chen, Antonia F. ; Grant, Matthew ; Saleh‐mghir, Azzam ; Mirza, Mohsin ; Stutzman, Bradley ; Vergari, Claudio ; Yue, James ; Anzala, Nelson ; Bonnot, Dorian ; Albac, Sandrine ; Bouloussaman ; Croisier, Delphine ; Brigham & Women’s Hospital Boston (BWH) ; Harvard Medical School Boston (HMS) ; Infection et inflammation (2I) ; Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM) ; Institut de Biomécanique Humaine Georges Charpak (IBHGC) ; Université Sorbonne Paris Nord-Arts et Métiers Sciences et Technologies ; HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM) ; Dijon, CHU ; Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon) ; Service de Maladies Infectieuses et Tropicales CHU Dijon ; Vivexia S.A.R.L. Gemeaux |
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Zeitschrift: | ISSN: 1554-527X ; Journal of Orthopaedic Research ; https://hal.science/hal-04195338 ; Journal of Orthopaedic Research, 2023, ⟨10.1002/jor.25638⟩, 2023 |
Veröffentlichung: | HAL CCSD, 2023 |
Medientyp: | academicJournal |
DOI: | 10.1002/jor.25638 |
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