Establishment of SV40 Large T-Antigen-Immortalized Yak Rumen Fibroblast Cell Line and the Fibroblast Responses to Lipopolysaccharide
In: Toxins, Vol 15, Iss 537, p 537 (2023, Jg. 15 (2023), Heft 537, p 537
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Zugriff:
The yak lives in harsh alpine environments and the rumen plays a crucial role in the digestive system. Rumen-associated cells have unique adaptations and functions. The yak rumen fibroblast cell line (SV40T-YFB) was immortalized by introducing simian virus 40 large T antigen (SV40T) by lentivirus-mediated transfection. Further, we have reported the effects of lipopolysaccharide (LPS) of different concentrations on cell proliferation, extracellular matrix (ECM), and proinflammatory mediators in SV40T-YFB. The results showed that the immortalized yak rumen fibroblast cell lines were identified as fibroblasts that presented oval nuclei, a fusiform shape, and positive vimentin and SV40T staining after stable passage. Chromosome karyotype analysis showed diploid characteristics of yak (n = 60). LPS at different concentrations inhibited cell viability in a dose-dependent manner. SV40T-YFB treated with LPS increased mRNA expression levels of matrix metalloproteinases (MMP-2 and MMP-9), inflammatory cytokines (TNF-α, IL-1β, IL-6), and urokinase-type plasminogen activator system components (uPA, uPAR). LPS inhibits the expression of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2), plasminogen activator inhibitor-2 (PAI-2), fibronectin (FN), anti-inflammatory factor IL-10, and collagen I (COL I) in SV40T-YFB. Overall, these results suggest that LPS inhibits cell proliferation and induces ECM degradation and inflammatory response in SV40T-YFB.
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Establishment of SV40 Large T-Antigen-Immortalized Yak Rumen Fibroblast Cell Line and the Fibroblast Responses to Lipopolysaccharide
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Autor/in / Beteiligte Person: | Wang, Junmei ; Yue, Ziqi ; Che, Li ; Li, Hui ; Hu, Rui ; Shi, Liyuan ; Zhang, Xiaohong ; Zou, Huawei ; Peng, Quanhui ; Jiang, Yahui ; Wang, Zhisheng |
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Zeitschrift: | Toxins, Vol 15, Iss 537, p 537 (2023, Jg. 15 (2023), Heft 537, p 537 |
Veröffentlichung: | MDPI AG, 2023 |
Medientyp: | academicJournal |
ISSN: | 2072-6651 (print) |
DOI: | 10.3390/toxins15090537 |
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