Genome-wide profiling of transcribed enhancers during macrophage activation
In: Epigenetics & Chromatin ; https://epigeneticsandchromatin.biomedcentral.com/, 2017
Online
academicJournal
Zugriff:
Background: Macrophages are sentinel cells essential for tissue homeostasis and host defence. Owing to their plasticity, macrophages acquire a range of functional phenotypes in response to microenvironmental stimuli, of which M(IFN-γ) and M(IL-4/IL-13) are well known for their opposing pro- and anti-inflammatory roles. Enhancers have emerged as regulatory DNA elements crucial for transcriptional activation of gene expression. Results: Using cap analysis of gene expression and epigenetic data, we identify on large-scale transcribed enhancers in bone marrow-derived mouse macrophages, their time kinetics, and target protein-coding genes. We observe an increase in target gene expression, concomitant with increasing numbers of associated enhancers, and find that genes associated with many enhancers show a shift towards stronger enrichment for macrophage-specific biological processes. We infer enhancers that drive transcriptional responses of genes upon M(IFN-γ) and M(IL-4/IL-13) macrophage activation and demonstrate stimuli specificity of regulatory associations. Finally, we show that enhancer regions are enriched for binding sites of inflammation-related transcription factors, suggesting a link between stimuli response and enhancer transcriptional control. Conclusions: Our study provides new insights into genome-wide enhancer-mediated transcriptional control of macrophage genes, including those implicated in macrophage activation, and offers a detailed genome-wide catalogue of transcribed enhancers in bone marrow-derived mouse macrophages.
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Genome-wide profiling of transcribed enhancers during macrophage activation
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Autor/in / Beteiligte Person: | Denisenko, Elena ; Guler, Reto ; Mhlanga, Musa M ; Suzuki, Harukazu ; Brombacher, Frank ; Schmeier, Sebastian |
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Zeitschrift: | Epigenetics & Chromatin ; https://epigeneticsandchromatin.biomedcentral.com/, 2017 |
Veröffentlichung: | BioMed Central ; University of Cape Town ; Faculty of Health Sciences ; Division of Infectious Disease and HIV Med, 2017 |
Medientyp: | academicJournal |
DOI: | 10.1186/s13072-017-0158-9 |
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