DataSheet1_Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.pdf
2022
Online
unknown
Zugriff:
We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy. A tetrasaccharide core structure GlcNH 2 β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work. When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis. Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
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DataSheet1_Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.pdf
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Autor/in / Beteiligte Person: | Ooi, Kai-Eng ; Zhang, Xiu-Wen ; Kuo, Cheng-Yu ; Liu, Ying-Jia ; Yu, Ching-Ching |
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Veröffentlichung: | 2022 |
Medientyp: | unknown |
DOI: | 10.3389/fchem.2022.905105.s001 |
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