Additional file 6 of Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
2021
Bild
Zugriff:
Additional file 5. Figure S3. Effects of GPR55 modulators on osteoclast maturation. (a, b) Real-time PCR analysis of the differentiation markers (as indicated) in RAW264.7 cells treated with 15 ng/mL RANKL for 72 h, in the absence or presence of 1 µM soybean LPI alone or with GPR55 antagonists (0.5 µM ML-191; 0.5 µM CID16020046). (c) Real-time PCR analysis of Ctr in RAW264.7 cells interfered with non-targeting (si-NT) or Gpr55-targeting (si-GPR55) siRNAs, and subsequently treated with 15 ng/mL RANKL for 72 h in the absence or presence of 1 µM soybean LPI or 1 µM ML-184. The transcripts were quantified and normalised using β2-microglobulin expression, as the housekeeping gene. Data are expressed as proportions (%) of the corresponding control RANKL, as means ±SEM from at least three independent experiments. *p <0.05, **p <0.01 (Student’s t-tests). RANKL, RANKL-differentiated cells. CID, CID16020046.
Titel: |
Additional file 6 of Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
|
---|---|
Autor/in / Beteiligte Person: | Maria Giovanna Mosca (10704023) ; Maria Mangini (10704026) ; Stefania Cioffi (10704029) ; Pasquale Barba (591315) ; Stefania Mariggiò (10704032) |
Link: | |
Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.6084/m9.figshare.14490266.v1 |
Schlagwort: |
|
Sonstiges: |
|