Defining the transcriptome of MeV infected HAE with scRNA-seq.
2021
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Zugriff:
(A) The experimental design is shown schematically. MeV or mock-infected HAE (n = 10 human donors; MOI = 5) were sorted via FACS at day 3, 7, or 14 post-infection and gated for GFP expression. GFP+ and GFP- cells were collected from MeV-infected HAE. Control cells were sorted via FACS from mock infected cultures (referred to as Mock). Cells from all 10 donors were pooled within their treatment type and prepared for scRNA-seq (10x Genomics). In total, 30,743 cells were sequenced. We projected these cells in a Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) and color-coded them by their treatment group (B) and cell type (C). (D) The percentage of each cell type within each treatment group is shown.
Titel: |
Defining the transcriptome of MeV infected HAE with scRNA-seq.
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Autor/in / Beteiligte Person: | Camilla E. Hippee (11269169) ; Brajesh K. Singh (8032523) ; Andrew L. Thurman (11269172) ; Ashley L. Cooney (8543751) ; Alejandro A. Pezzulo (11269175) ; Roberto Cattaneo (363765) ; Patrick L. Sinn (8543754) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.ppat.1009458.g005 |
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