Actions of sex steroids on kisspeptin expression and other reproduction-related genes in the brain of the teleost fish European sea bass
In: ISSN: 0022-0949, 2016
academicJournal
Zugriff:
International audience ; Kisspeptins are well known as mediators of the coordinated communication between the brain-pituitary axis and the gonads in many vertebrates. To test the hypothesis that gonadal steroids regulate kiss1 and kiss2 mRNA expression in European sea bass (a teleost fish), we examined the brains of gonad-intact (control) and castrated animals, as well as castrated males (GDX) and ovariectomized females (OVX) that received testosterone (T) and estradiol (E2) replacement, respectively, during recrudescence. In GDX males, low expression of kiss1 mRNA is observed by in situ hybridization in the caudal hypothalamus (CH) and the mediobasal hypothalamus (MBH), although hypothalamic changes in kiss1 mRNA levels were not statistically different among the groups, as revealed by real-Time PCR. However, T strongly decreased kiss2 expression levels in the hypothalamus, which was documented in the MBH and the nucleus of the lateral recess (NRLd) in GDX T-Treated sea bass males. Conversely, it appears that E2 evokes lowkiss1mRNAin the CH, while there were cells expressing kiss2 in the MBH and NRLd in these OVX females. These results demonstrate that kisspeptin neurons are presumably sensitive to the feedback actions of sex steroids in the sea bass, suggesting that the MBH represents a major site for sex steroid actions on kisspeptins in this species. Also, recent data provide evidence that both positive and negative actions occur in key factors involved in sea bass reproductive function, including changes in the expression of gnrh-1/gonadotropin, cyp19b, er and ar genes and sex steroid and gonadotropin plasma levels in this teleost fish.
Titel: |
Actions of sex steroids on kisspeptin expression and other reproduction-related genes in the brain of the teleost fish European sea bass
|
---|---|
Autor/in / Beteiligte Person: | Alvarado, M.V. ; Servili, Arianna ; Molés, G. ; Gueguen, M.M. ; Carrillo, M. ; Kah, O. ; Felip, A. ; Instituto de Acuicultura de Torre la Sal (IATS) ; Consejo Superior de Investigaciones Cientificas España = Spanish National Research Council Spain (CSIC) ; Centro de Investigaciones Biológicas (CSIC) ; Laboratoire de Physiologie des Invertébrés (LPI) ; Physiologie Fonctionnelle des Organismes Marins (PFOM) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ; Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) ; Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) ; Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ; Institut de recherche en santé, environnement et travail (Irset) ; Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique EHESP (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) ; Ministerio de Ciencia e Innovación ; CSD2007-00002, Ministerio de Economía y Competitividad ; Valenciana, Generalitat ; European Project: 222719,EC:FP7:KBBE,FP7-KBBE-2007-2A,LIFECYCLE(2009) |
Link: | |
Zeitschrift: | ISSN: 0022-0949, 2016 |
Veröffentlichung: | HAL CCSD ; The Company of Biologists, 2016 |
Medientyp: | academicJournal |
DOI: | 10.1242/jeb.137364 |
Schlagwort: |
|
Sonstiges: |
|