Stimulation by DIF causes an increase of intracellular Ca 2+ in Dictyostelium discoideum
In: Azhar, M.; Kennady, P. K.; Pande, G.; Nanjundiah, Vidyanand (1997) Stimulation by DIF causes an increase of intracellular Ca2+ in Dictyostelium discoideum Experimental Cell Research, 230 (2), 1997, S. 403-406
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Zugriff:
Using fluorescence-activated cell sorting (FACS), we have studied the effect of the differentiation-inducing factor (DIF) on cellular Ca 2+ inDictyostelium discoideum.We have shown previously that freshly starved or postaggregation amoebae are heterogenous with respect to the amounts of cellular Ca 2+ that they contain; the L or "low Ca 2+ " class exhibits a prespore tendency and the H or "high Ca 2+ " class exhibits a prestalk tendency. Upon adding DIF, within 2 min there is an approximately twofold increase in the relative fraction of amoebae falling in the H class. A major part of the increase is caused by Ca 2+ influx from the extracellular medium. Therefore a rise in the level of cellular Ca 2+ is an early step in the signal transduction pathway following stimulation by DIF. Also, in parallel with the cellular heterogeneity in respect of Ca 2+ content, there is a heterogeneity in the response to DIF, which appears to be restricted to L cells.
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Stimulation by DIF causes an increase of intracellular Ca 2+ in Dictyostelium discoideum
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Autor/in / Beteiligte Person: | Azhar, M. ; Kennady, P. K. ; Pande, G. ; Nanjundiah, Vidyanand |
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Zeitschrift: | Azhar, M.; Kennady, P. K.; Pande, G.; Nanjundiah, Vidyanand (1997) Stimulation by DIF causes an increase of intracellular Ca2+ in Dictyostelium discoideum Experimental Cell Research, 230 (2), 1997, S. 403-406 |
Veröffentlichung: | Elsevier Science, 1997 |
Medientyp: | academicJournal |
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