Capillary Gas Chromatography with Four Different Detectors for Dinitroaniline Herbicides in Human Body Fluids
Oxford University Press, 1995
academicJournal
Zugriff:
A simple and rapid method for extraction of seven dinitroaniline herbicides from human whole blood and urine using Sep-Pak C 18 cartridges is presented. After mixing with distilled water, whole blood and urine samples containing the herbicides were loaded on Sep-Pak C 18 cartridges and eluted with chloroform-methanol (9:1). The recovery of the seven compounds that were added to whole blood and urine was more than 91%. The herbicides were detected by capillary gas chromatography (GC) with electron-capture detection (ECD), surface-ionization detection (SID), nitrogen-phosphorus detection (NPD), and flame-ionization detection (FID). GC-ECD generally gave the highest sensitivity for all herbicides, whereas GC-FID gave the lowest sensitivity. Isopropalin could be detected by GC-SID with a sensitivity comparable with that of GC-ECD; high sensitivity could also be obtained for isopropalin, pendimethalin, and nitralin using GC-NPD. The calibration curve for the seven herbicides measured by GC-ECD showed linearity in the range of 0.047–3.0 pmol on-column. The detection limits of the herbicides were 24–45 fmol on-column for whole blood and 19–40 fmol on-column for urine.
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Capillary Gas Chromatography with Four Different Detectors for Dinitroaniline Herbicides in Human Body Fluids
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Autor/in / Beteiligte Person: | Kumazawa, Takeshi ; Sato, Keizo ; Seno, Hiroshi ; Ishii, Akira ; Suzuki, Osamu |
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Veröffentlichung: | Oxford University Press, 1995 |
Medientyp: | academicJournal |
DOI: | 10.1093/jat/19.2.95 |
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