The Analysis of Genetic Variability of Norway Spruce (Picea abies (L.) Karst.) Subpopulation at the Igman Mountain ; Genetička diferenciranost subpopulacija obične smreke (Picea abies (L.) Karst.) na planini Igman

In this study we analyzed the genetic structure of two autochthonous subpopulations of Norway spruce in the Mountain of Igman by usage of isoenzyme markers. We collected the material for the analysis in two separate plant communities. The subpopulation Igman – A is represented by fir-tree and spruce forest with randomly distributed white pine trees (Abieti-Piceetum illyricum Stef. 1960) while the Igman – B subpopulation is represented through the spruce tree of frosty type in the mountain area (Piceetum montanum s.lat. (Fuk. et Stef., 1958, emend. Horv. et al., 1974)). Between the subpopulations there is a 150 m difference in altitude. We analyzed the following systems of Acotinase (Aco-A), Glutamate dehydrogenase (Gdh-A), Glutamate oxaloacetate transaminase (Got-A, Got-B, Got-C), Izocitrate dehidrogenase (Idh-A, Idh-B), Leucine aminopeptidase (Lap-B), Malate dehydrogenase (Mdh-A, Mdh-B, Mdh-C), Menadione reductase (Mnr-A, Mnr-C), Phosphoglucose isomerase (Pgi-B), Phosphoglucomutase (Pgm-A), Shikimate dehydrogenase (Skdh-A), 6-Phosphogluconate dehydrogenase (6-Pgdh-A, 6-Pgdh-B, 6-Pgdh-C) and Fluorescentesterase (Fest-B). The frequency of the allele and the frequency of genotypes show diversity between subpopulations. The Allele differentiation was most evident at loci Got-C, 6-Pgdh-A. In the sample of the Igman – A subpopulation the frequency of the allele Aco-A2 was 7 % lower, and the frequency of 6-Pgdh-A2 7 % higher than in the sample from Igman – B subpopulation. The genotype subpopulations are most explicitly differentiated at loci Fest-B, Got-C, Lap-B, Mdh-C, Mnr-A, Mnr-C, Pgi-B, 6-Pgdh-A, 6-Pgdh-B, 6-Pgdh-C. If the Igman – A subpopulation is compared with Igman – B subpopulation, we have 8–14% higher frequency of homozygote: Got-C44 (52 % vs. 44 %), Fest22 (90 % vs. 80 %), Mnr-A22 (12 % vs. 4 %), Mnr-C22 (94 % vs. 82 %), 6-Pgdh-A22 (94 % vs. 80 %), 6-Pgdh-C22 (42 % vs. 30 %) and from 10–14 % higher heterozygote frequency for gene loci: Lap-B46 (12 % vs. 0 %), Pgi-B23 (52 % vs. 42 %), 6-Pgdh-B25 (54 % vs. ...