Recurrent breakpoints in 14q32.13/TCL1A region in mature B-cell neoplasms with villous lymphocytes
In: Leukemia and Lymphoma, Vol. 53, no.12, p. 2449-2455 (2012, 2012
Online
academicJournal
Zugriff:
The genetic background of mature B-cell neoplasms with villous lymphocytes is poorly understood. We identified a novel breakpoint region at 14q32.13 that was rearranged together with IGH/14q32.33 in four cases of BRAF/V600E-negative leukemia/lymphoma with villous lymphocytes carrying either t(14;14)(q32.13;q32.33) (three patients) or del(14)(q32.13q32.33) (one patient). The 14q32.13 breakpoints were mapped by fluorescence in situ hybridization (FISH) in the region harboring the TCL1A/TCL1B/TCL6 genes, known to be affected by TCRA/D-mediated t(14;14)(q11;q32)/inv(14)(q11q32) occurring in T-cell leukemia/lymphoma. To identify the target of t(14;14)(q32.13; q32.33) and del(14)(q32.13q32.33), quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 25 candidate genes located centromerically and telomerically to the 14q32.13 breakpoint was performed. Any of the analyzed genes was commonly overexpressed in the presented cases. Of note, up-regulated transcription of TCL1A was observed in two cases. In summary, we provide evidence that IGH-mediated chromosomal aberrations affecting the 14q32.13/TCL1A-TCL6 region are recurrent in mature B-cell neoplasms with villous lymphocytes. Despite extensive qRT-PCR studies, molecular consequences of these novel aberrations remain elusive.
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Recurrent breakpoints in 14q32.13/TCL1A region in mature B-cell neoplasms with villous lymphocytes
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Autor/in / Beteiligte Person: | Urbankova, Helena ; Baens, Mathijs ; Michaux, Lucienne ; Tousseyn, Thomas ; Rack, Katrina ; Katrincsakova, Beata ; Ferreiro, Julio Finalet ; van Loo, Peter ; de Kelver, Wim ; Dierickx, Daan ; Demuynck, Hilde ; Delannoy, André ; Verschuere, Johan ; Jarošová, Marie ; de Wolf-Peeters, Chris ; Vandenberghe, Peter ; Wlodarska, Iwona ; UCL - SSS/IREC - Institut de recherche expérimentale et clinique ; UCL - (SLuc) Service d'hématologie |
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Zeitschrift: | Leukemia and Lymphoma, Vol. 53, no.12, p. 2449-2455 (2012, 2012 |
Veröffentlichung: | Informa Healthcare, 2012 |
Medientyp: | academicJournal |
ISSN: | 1042-8194 (print) ; 1029-2403 (print) |
DOI: | 10.3109/10428194.2012.690098 |
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