Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation
In: Abraham, Irene; Tyagi, Jaya Sivaswami; Gottesman, Michael M. (1982) Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation Somatic Cell and Molecular Genetics, 1982, S. 23-39
academicJournal
Zugriff:
We have transferred DNA to Chinese hamster ovary (CHO) cells by DNA-mediated transformation. CHO tk − cells were transformed with the cloned gene for herpes simplex virus thymidine kinase (HSV-tk) and were found to have a 50-fold lower frequency of transformation than mouse Ltk − cells at the same DNA dosage. By altering the amount of tk gene and carrier DNA present, frequencies of up to 5 × 10 −5 were obtained. CHO HSV- tk + transformants were very stable, and in several clones the HSV-tk gene copies integrated in higher-molecular-weight DNA. These cells also exhibited cotransformation for unselected markers. CHO lines were also transformed at a frequency of 10 −4 with the bacterial gene Ecogpt in a SV40-pBR322 vector. CHO tk − cells could be transformed at a frequency of 10 −7 with cellular DNA isolated from CHO tk + cells. CHO cells offer a well-defined genetic system within which to transfer either cloned or whole cellular DNAs.
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Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation
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Autor/in / Beteiligte Person: | Abraham, Irene ; Tyagi, Jaya Sivaswami ; Gottesman, Michael M. |
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Zeitschrift: | Abraham, Irene; Tyagi, Jaya Sivaswami; Gottesman, Michael M. (1982) Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation Somatic Cell and Molecular Genetics, 1982, S. 23-39 |
Veröffentlichung: | Springer, 1982 |
Medientyp: | academicJournal |
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