Transmission of CWD prions from Norwegian reindeer to mice expressing E226 and Q226 PrP.

Survival curves of TgE226 (orange circles), TgQ226 (magenta circles), GtE226 (orange squares), and GtQ226 mice (magenta squares) following transmission of CWD prions from the CNS or LRS of three Norwegian reindeer CWD isolates, referred to as R-NO1, R-NO2, and R-NO3. A ., primary transmission (p1) of prions in R-NO1 brain homogenates; B ., p1 of prions in R-NO2 brain homogenates; C ., p1 of prions in R-NO3 brain homogenates; D ., second passage (p2) of prions in brain homogenates of diseased TgQ226 mice infected with R-NO1; E ., p2 of prions in brain homogenates of diseased TgQ226 mice infected with R-NO2; F ., p2 of prions in brain homogenates of diseased GtQ226 mice infected with R-NO1; G ., p1 of prions in R-NO2 lymphoid tissue homogenates; H ., p1 of prions in R-NO3 lymphoid tissue homogenates. Mice from which brains were used for serial transmissions are indicated by arrows. Dotted squares, Gt mice receiving CWD prions previously passaged in GtQ226 mice. Inserts show median times to disease and statistical differences between different pairs of transmissions indicated by brackets.