Additional file 4 of Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
2021
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Zugriff:
Additional file 3. Figure S1. LPI-induced signalling is independent of GPR55 overexpression in HEK293T and HeLa cells. (a) FACS analysis with an anti-HA antibody of HEK293T cells transfected with empty vector (pcDNA3) or the vector coding for GPR55 wild-type (HA-GPR55) or mutants (as indicated). (b, c) Twenty-four hours after transfection, these HEK293T cells were serum deprived for 4 h, and then stimulated with 10 µM LPI for the indicated times. Western blotting for phosphorylated (p-ERK1/2) and total ERK1/2 are shown, from a representative experiment of three independent ones. (d). HeLa cells transfected with empty vector (pcDNA3.1) or the vector coding for the construct ss-3×HA-GPR55 (ssGPR55). Twenty-four hours after transfection, the cells were serum deprived for 2 h, and then stimulated with 10 µM soybean LPI for the indicated times. Western blotting for phosphorylated (p-AKT, p-ERK1/2) and total AKT and ERK1/2 are shown, from a representative experiment of three independent ones.
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Additional file 4 of Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
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Autor/in / Beteiligte Person: | Maria Giovanna Mosca (10704023) ; Maria Mangini (10704026) ; Stefania Cioffi (10704029) ; Pasquale Barba (591315) ; Stefania Mariggiò (10704032) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.6084/m9.figshare.14490260.v1 |
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