ETFDH is required for RQ-dependent metabolism.
2021
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Zugriff:
(a) Schematic showing role of QDHs in the electron transport chain in UQ and RQ-dependent metabolism. (b) Requirement for QDHs in RQ-dependent metabolism. RNAi knockdown of QDH genes was performed by feeding dsRNA-expressing bacterial strains to L1 worms until they developed to fertile adults. Their L1 progeny were subjected to 15 h of 200 μM KCN treatment followed by a 3 h recovery (see Materials and methods ). An RNAi clone targeting GFP was used as a negative control. Data show movement scores after 3 h recovery and represent biological replicates, with 2–3 technical replicates in each. (c) Conserved divergent residues from host (blue), C . elegans (yellow), parasite (red) and mollusk/annelids (green) were identified using S3Det [ 38 ] with a cutoff of 2 (see Materials and methods ). Residues are numbered according to C . elegans . Phe437 (arrow) is conserved in RQ-containing species but is not present in hosts. (d) Crystal structure of porcine ETFDH (PDB: 2GMH) in complex with UQ5 (orange) as visualized in Chimera [ 85 ] with C . elegans sequence (pink residues) and H . sapiens sequence (cyan residues) threaded over by homology (residues from (c) circled in red). The Phe437Cys residue (green) is located near the quinone binding site [ 89 ] where both RQ and UQ must be able to bind in C . elegans and parasites.
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ETFDH is required for RQ-dependent metabolism.
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Autor/in / Beteiligte Person: | Margot J. Lautens (11767950) ; June H. Tan (4582975) ; Xènia Serrat (7522331) ; Samantha Del Borrello (5447900) ; Michael R. Schertzberg (11767953) ; Andrew G. Fraser (11767956) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pntd.0009991.g004 |
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