Isotopologue distribution patterns for metabolites in or directly related to the TCA cycle of C . burnetii wildtype, 0823 ::Tn mutant and 0823 ::Tn pFLAG-CBU0823 complemented strains.
2021
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Zugriff:
C . burnetii incubated with [ 13 C]glucose supplemented ACCM-2 for 7 days before GC-MS analysis to determine 13 C incorporated into the extracted metabolites. Mean isotopologue distribution of each metabolite detected, corrected for natural isotopic abundance with 0% unlabelled biomass, was graphed using DExSI for each strain. Error bars represent standard deviation from five biological replicates. Solid lines represent a single enzyme and dotted lines represent multiple enzymes. The blue line denotes the activity of CBU0823 as a ME. The green line denotes CBU1241 as an MDH. The orange line represents an unestablished conversion of pyruvate to lactate by an LDH-type reaction. PEP = phosphoenolpyruvic acid. (TIF)
Titel: |
Isotopologue distribution patterns for metabolites in or directly related to the TCA cycle of C . burnetii wildtype, 0823 ::Tn mutant and 0823 ::Tn pFLAG-CBU0823 complemented strains.
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Autor/in / Beteiligte Person: | Janine Hofmann (11273418) ; Mebratu A. Bitew (11273421) ; Miku Kuba (11273424) ; David P. De Souza (2793088) ; Hayley J. Newton (8692020) ; Fiona M. Sansom (8893082) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0255925.s005 |
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