Crz signaling influences osmotic and ionic stresses.
2021
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Zugriff:
(A) Crz 1 -GAL4 driven Crz-RNAi (1) causes a significant decrease in anti-Crz staining (corrected total cell fluorescence, CTCF) in the brains of adult Drosophila (**** p < 0.0001 as assessed by unpaired t- test). (B) Crz knockdown results in a significant delay in recovery from chill-coma and (C) increased survival under ionic stress. For B and C , data are presented as survival curves (**** p < 0.0001, as assessed by Log-rank (Mantel-Cox) test). Crz peptide injections in vivo influence (D, E) chill-coma recovery and (F) desiccation survival. (D) Low dose (10 -9 M) of injected Crz has no effect whereas a high dose (10 -6 M) of Crz delays chill-coma recovery (** p < 0.01, *** p < 0.001 as assessed by two-way ANOVA). (E) A non-active exogenous peptide (10 -6 M), non-amidated Aedes adipokinetic hormone/corazonin-related peptide (naACP), has no effect on chill-coma recovery (** p < 0.01 as assessed by two-way ANOVA). In D and E , the y-axes (Time) represents the chill-coma recovery (CCR) time. (F) We used a Cox proportional hazards model to test whether injection of 10 -6 M Crz (red lines) influences the rate of death under desiccation conditions compared to saline injection (grey lines), while accounting for any time-effects (different time points are indicated using circle, triangle and square symbols). Crz injection increases the risk of death by 40% compared to control flies. This difference is statistically significant at p = 0.0003. Two independent trials for E and three independent trials for D and F were performed. These trials are indicated using different symbols (circle, triangle and square).
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Crz signaling influences osmotic and ionic stresses.
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Autor/in / Beteiligte Person: | Meet Zandawala (491178) ; Thomas Nguyen (2026324) ; Marta Balanyà Segura (10271484) ; Helena A. D. Johard (10271487) ; Mirjam Amcoff (8036318) ; Christian Wegener (21851) ; Jean-Paul Paluzzi (510789) ; Dick R. Nässel (7894226) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pgen.1009425.g001 |
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