Connexin 30 controls astroglial polarization during postnatal brain development
In: ISSN: 0950-1991, 2018
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Zugriff:
International audience ; Astrocytes undergo intense morphological maturation during development, changing from individual sparsely branched cells to polarized and tremendously ramified cells. Connexin 30, an astroglial gap-junction channel-forming protein expressed postnatally, regulates in situ the extension and ramification of astroglial processes. However, the involvement of connexin 30 in astroglial polarization, which is known to control cell morphology, remains unexplored. We found that connexin 30, independently of gap-junction-mediated intercellular biochemical coupling, alters the orientation of astrocyte protrusion, centrosome and Golgi apparatus during polarized migration in an in vitro wound-healing assay. Connexin 30 sets the orientation of astroglial motile protrusions via modulation of the laminin/β1 integrin/ Cdc42 polarity pathway. Connexin 30 indeed reduces laminin levels, inhibits the redistribution of the β1-integrin extracellular matrix receptors, and inhibits the recruitment and activation of the small Rho GTPase Cdc42 at the leading edge of migrating astrocytes. In vivo, connexin 30, the expression of which is developmentally regulated, also contributes to the establishment of hippocampal astrocyte polarity during postnatal maturation. This study thus reveals that connexin 30 controls astroglial polarity during development.
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Connexin 30 controls astroglial polarization during postnatal brain development
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Autor/in / Beteiligte Person: | Ghézali, Grégory ; Calvo, Charles-Félix ; Pillet, Laure-Elise ; Llense, Flora ; Ezan, Pascal ; Pannasch, Ulrike ; Bemelmans, Alexis-Pierre ; Etienne-Manneville, Sandrine ; Rouach, Nathalie ; Sorbonne Université - Faculté de Médecine (SU FM) ; Sorbonne Université (SU) ; Centre interdisciplinaire de recherche en biologie (CIRB) ; MemoLife, Labex ; École normale supérieure - Paris (ENS-PSL) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris) ; Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL) ; Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) ; Université Paris Descartes - Paris 5 (UPD5) ; Polarité cellulaire, Migration et Cancer - Cell Polarity, Migration and Cancer ; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS) ; Laboratoire des Maladies Neurodégénératives - UMR 9199 (LMN) ; Molecular Imaging Research Center Fontenay-aux-Roses (MIRCEN) ; Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB) ; Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) ; This work was supported by grants from the European Research Council (consolidator grant 683154 to N.R.), from the Paris 6 University doctoral school ED3C and Labex Memolife to G.G., from the Paris Descartes university doctoral school Bio Sorbonne Paris Cité to L.-E. P., from the Centre National de la Recherche Scientifique, and from the Institut Pasteur to S.E.-M. and F.L. ; ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010) ; European Project: 683154,H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC),AstroWireSyn(2016) |
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Zeitschrift: | ISSN: 0950-1991, 2018 |
Veröffentlichung: | HAL CCSD ; Company of Biologists, 2018 |
Medientyp: | academicJournal |
DOI: | 10.1242/dev.155275 |
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