Cross-reactive inhibitory antibody and memory B cell responses to variant strains of Duffy binding protein II at post-Plasmodium vivax infection
In: PLOS ONE, Jg. 17 (2022), Heft 10, S. e0276335
academicJournal
Zugriff:
Duffy binding protein region II (DBPII) is considered a strong potential vaccine candidate of blood-stage P . vivax . However, the highly polymorphic nature of this protein often misdirects immune responses, leading them to be strain-specific. Details of cross-reactive humoral immunity to DBPII variants have therefore become an important focus for the development of broadly protective vaccines. Here, cross-reactive humoral immunity against a panel of Thai DBPII variants (DBL-THs) was demonstrated in immunized BALB/c mice and P . vivax patients, by in vitro erythrocyte-binding inhibition assay. Sera from immunized animals showed both strain-transcending (anti-DBL-TH2 and -TH4) and strain-specific (anti-DBL-TH5, -TH6 and -TH9) binding to DBL-TH variants. Using anti-DBL-TH sera at 50% inhibitory concentration (IC50) of the homologous strain, anti-DBL-TH2 sera showed cross inhibition to heterologous DBL-TH strains, whereas anti-DBL-TH5 sera exhibited only strain-specific inhibition. In P . vivax patients, 6 of 15 subjects produced and maintained cross-reactive anti-DBL-TH inhibitory antibodies through the 1-year post-infection timepoint. Cross-reactive memory B cell (MBC) responses to DBL-TH variants were analyzed in subjects recovered from P . vivax infection (RC). The plasma samples from 5 RC subjects showed broad inhibition. However, MBC-derived antibodies of these patients did not reveal cross-inhibition. Altogether, broadly anti-DBP variant inhibitory antibodies developed and persisted in P . vivax infections. However, the presence of cross-reactive anti-DBL-TH inhibitory function post-infection was not related with MBC responses to these variants. More detailed investigation of long-lasting, broadly protective antibodies to DBPII will guide the design of vivax malaria vaccines.
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Cross-reactive inhibitory antibody and memory B cell responses to variant strains of Duffy binding protein II at post-Plasmodium vivax infection
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Autor/in / Beteiligte Person: | Thawornpan, Pongsakorn ; Changrob, Siriruk ; Kochayoo, Piyawan ; Wangriatisak, Kittikorn ; Ntumngia, Francis B. ; De, Sai Lata ; Han, Eun-Taek ; Adams, John H. ; Chootong, Patchanee ; Braga, Érika Martins ; University, Mahidol |
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Zeitschrift: | PLOS ONE, Jg. 17 (2022), Heft 10, S. e0276335 |
Veröffentlichung: | Public Library of Science (PLoS), 2022 |
Medientyp: | academicJournal |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0276335 |
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