SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma
In: BMC Genomics, Jg. 19 (2018), Heft 1, S. 1-12
Online
academicJournal
Zugriff:
Background Brahma (BRM) is the only catalytic subunit of the SWI/SNF chromatin-remodeling complex of Drosophila melanogaster. The function of SWI/SNF in transcription has long been attributed to its ability to remodel nucleosomes, which requires the ATPase activity of BRM. However, recent studies have provided evidence for a non-catalytic function of BRM in the transcriptional regulation of a few specific genes. Results Here we have used RNA-seq and ChIP-seq to identify the BRM target genes in S2 cells, and we have used a catalytically inactive BRM mutant (K804R) that is unable to hydrolyze ATP to investigate the magnitude of the non-catalytic function of BRM in transcription regulation. We show that 49% of the BRM target genes in S2 cells are regulated through mechanisms that do not require BRM to have an ATPase activity. We also show that the catalytic and non-catalytic mechanisms of SWI/SNF regulation operate on two subsets of genes that differ in promoter architecture and are linked to different biological processes. Conclusions This study shows that the non-catalytic role of SWI/SNF in transcription regulation is far more prevalent than previously anticipated and that the genes that are regulated by SWI/SNF through ATPase-dependent and ATPase-independent mechanisms have specialized roles in different cellular and developmental processes.
Titel: |
SWI/SNF regulates half of its targets without the need of ATP-driven nucleosome remodeling by Brahma
|
---|---|
Autor/in / Beteiligte Person: | Jordán-Pla, Antonio ; Yu, Simei ; Waldholm, Johan ; Källman, Thomas ; Ann-Kristin Östlund Farrants ; Visa, Neus |
Link: | |
Zeitschrift: | BMC Genomics, Jg. 19 (2018), Heft 1, S. 1-12 |
Veröffentlichung: | BMC, 2018 |
Medientyp: | academicJournal |
ISSN: | 1471-2164 (print) |
DOI: | 10.1186/s12864-018-4746-2 |
Schlagwort: |
|
Sonstiges: |
|