Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge
In: ISSN: 0142-9612 ; Biomaterials ; https://hal.science/hal-03875857 ; Biomaterials, 2023, 292, pp.121907. ⟨10.1016/j.biomaterials.2022.121907⟩, 2023
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Zugriff:
International audience ; The ongoing SARS-CoV-2 pandemic represents a brutal reminder of the continual threat of mucosal infectious diseases. Mucosal immunity may provide robust protection at the predominant sites of SARS-CoV-2 infection. However, it remains unclear whether respiratory mucosal administration of DNA vaccines could confer protective immune responses against SARS-CoV-2 challenge due to insurmountable barriers posed by the airway. Here, we applied self-assembled peptide-poloxamine nanoparticles with mucus-penetrating properties for pulmonary inoculation of a COVID-19 DNA vaccine (pSpike/PP-sNp). The pSpike/PP-sNp not only displays superior gene transfection and favorable biocompatibility in the mouse airway, but also promotes a tripartite immunity consisting of systemic, cellular, and mucosal immune responses that are characterized by mucosal IgA secretion, high levels of neutralizing antibodies, and resident memory phenotype T-cell responses in the lungs of mice. Most importantly, immunization with pSpike/PP-sNp completely eliminates SARS-CoV-2 infection in both upper and lower respiratory tracts and enables 100% survival rate of mice following lethal SARS-CoV-2 challenge. Our findings indicate PP-sNp is a promising platform in mediating DNA vaccines to elicit all-around mucosal immunity against SARS-CoV-2.
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Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge
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Autor/in / Beteiligte Person: | Sun, Si ; Li, Entao ; Zhao, Gan ; Tang, Jie ; Zuo, Qianfei ; Cai, Larry ; Xu, Chuanfei ; Sui, Cheng ; Ou, Yangxue ; Liu, Chang ; Li, Haibo ; Ding, Yuan ; Li, Chao ; Lu, Dongshui ; Zhang, Weijun ; Luo, Ping ; Cheng, Ping ; Gao, Yuwei ; Tu, Changchun ; Pitard, Bruno ; Rosenecker, Joseph ; Wang, Bin ; Liu, Yan ; Zou, Quanming ; Guan, Shan ; The Third Military Medical University ; Chinese Academy of Agricultural Sciences (CAAS) ; Advaccine (Suzhou) Biopharmaceuticals Co., Ltd Suzhou, China (ABC) ; The University of Queensland (UQ All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations ) ; Immunology and New Concepts in ImmunoTherapy (INCIT) ; Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Universitaire de Nantes (CHU Nantes)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) ; Ludwig-Maximilians University Munich (LMU) ; Fudan University Shanghai |
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Zeitschrift: | ISSN: 0142-9612 ; Biomaterials ; https://hal.science/hal-03875857 ; Biomaterials, 2023, 292, pp.121907. ⟨10.1016/j.biomaterials.2022.121907⟩, 2023 |
Veröffentlichung: | HAL CCSD ; Elsevier, 2023 |
Medientyp: | academicJournal |
DOI: | 10.1016/j.biomaterials.2022.121907 |
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