Assessment of Wild Mustard (Sinapis arvensis L.) Resistance to ALS-inhibiting Herbicides
In: https://www.intechopen.com/books/4787; (2015)
Buch
Zugriff:
There is an urgent need for rapid, accurate, and economical screening tests that can determine if weeds surviving a herbicide application are resistant. This chapter describes development and application of a simple root length bioassay technique for detection of wild mustard (Sinapis arvensis L.) resistance to ALS-inhibiting herbicides. This bioassay was performed in 2-oz WhirlPak® bags filled with 50 g of soil wetted to 100% moisture content at field capacity. Wild mustard seeds were pre-germinated in darkness in Petri dishes lined with moist filter paper for 2 days. Six seeds with well-developed radicles were planted in the non-treated soil and in soil with added herbicide, and plants were grown in a laboratory under fluorescent lights. After 4 days of growth, WhirlPak® bags were cut open, soil was washed away, intact plants were removed, and root length was measured with a ruler. The concentration of each herbicide in soil at which a significant root inhibition of susceptible biotype, but no root inhibition of a resistant biotype occurred was selected. Susceptibility/resistance of wild mustard populations was estimated by calculating the percentage of uninhibited roots of plants grown in the herbicide-treated soil as compared to the plants grown in the non-treated soil.
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Assessment of Wild Mustard (Sinapis arvensis L.) Resistance to ALS-inhibiting Herbicides
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Autor/in / Beteiligte Person: | Szmigielski, Anna M. ; Schoenau, Jeff J. ; Beckie, Hugh J. |
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Quelle: | https://www.intechopen.com/books/4787; (2015) |
Veröffentlichung: | IntechOpen, 2015 |
Medientyp: | Buch |
ISBN: | 978-953-51-2217-3 (print) ; 953-51-2217-7 (print) |
DOI: | 10.5772/61550 |
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