Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity
In: ISSN: 2041-1723, 2021
Online
academicJournal
Zugriff:
International audience ; Basal progenitors (BPs), including intermediate progenitors and basal radial glia, are generated from apical radial glia and are enriched in gyrencephalic species like humans, contributing to neuronal expansion. Shortly after generation, BPs delaminate towards the subventricular zone, where they further proliferate before differentiation. Gene expression alterations involved in BP delamination and function in humans are poorly understood. Here, we study the role of LGALS3BP, so far known as a cancer biomarker, which is a secreted protein enriched in human neural progenitors (NPCs). We show that individuals with LGALS3BP de novo variants exhibit altered local gyrification, sulcal depth, surface area and thickness in their cortex. Additionally, using cerebral organoids, human fetal tissues and mice, we show that LGALS3BP regulates the position of NPCs. Single-cell RNA-sequencing and proteomics reveal that LGALS3BP-mediated mechanisms involve the extracellular matrix in NPCs’ anchoring and migration within the human brain. We propose that its temporal expression influences NPCs’ delamination, corticogenesis and gyrification extrinsically.
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Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity
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| Autor/in / Beteiligte Person: | Kyrousi, Christina ; O’neill, Adam, C ; Brazovskaja, Agnieska ; He, Zhisong ; Kielkowski, Pavel ; Coquand, Laure ; Di Giaimo, Rossella ; D’ Andrea, Pierpaolo ; Belka, Alexander ; Forero Echeverry, Andrea ; Mei, Davide ; Lenge, Matteo ; Cruceanu, Cristiana ; Buchsbaum, Isabel, y ; Khattak, Shahryar ; Fabien, Guimiot ; Binder, Elisabeth ; Elmslie, Frances ; Guerrini, Renzo ; Baffet, Alexandre, D ; Sieber, Stephan, A ; Treutlein, Barbara ; Robertson, Stephen, P ; Cappello, Silvia ; Max Planck Institute of Psychiatry ; Max-Planck-Gesellschaft ; National and Kapodistrian University of Athens (NKUA) ; University of Otago Dunedin, Nouvelle-Zélande ; Max Planck Institute for Evolutionary Anthropology Leipzig ; Department of Biosystems Science and Engineering ETH Zürich (D-BSSE) ; Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology Zürich (ETH Zürich) ; Center for Integrated Protein Science (CIPSM) ; Technische Universität Munchen - Technical University Munich - Université Technique de Munich (TUM)-Helmholtz Zentrum München = German Research Center for Environmental Health-Ludwig Maximilian University Munich = Ludwig Maximilians Universität München (LMU) ; Ludwig Maximilian University Munich = Ludwig Maximilians Universität München (LMU) ; Optimisation thérapeutique en Neuropsychopharmacologie (OPTeN (UMR_S_1144 / U1144)) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité) ; Institut Curie Paris ; University of Naples Federico II = Università degli studi di Napoli Federico, II ; Università degli Studi di Firenze = University of Florence (UniFI) ; Technische Universität Dresden = Dresden University of Technology (TU Dresden) ; Royal College of Surgeons in Ireland (RCSI) ; Hôpital Robert Debré ; University of London London |
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| Zeitschrift: | ISSN: 2041-1723, 2021 |
| Veröffentlichung: | HAL CCSD ; Nature Publishing Group, 2021 |
| Medientyp: | academicJournal |
| DOI: | 10.1038/s41467-021-26447-w |
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