Leukocyte Receptor Tyrosine Kinase interacts with secreted midkine to promote survival of migrating neural crest cells
In: Development, 145(20), Art. No. dev164046, (2018-10-24, 2018
academicJournal
Zugriff:
Neural crest cells migrate long distances throughout the embryo and rely on extracellular signals that attract, repel and/or stimulate survival to ensure proper contribution to target derivatives. Here, we show that leukocyte receptor tyrosine kinase (LTK), an ALK-type receptor tyrosine kinase, is expressed by neural crest cells during early migratory stages in chicken embryos. Loss of LTK in the cranial neural crest impairs migration and results in increased levels of apoptosis. Conversely, midkine, previously proposed as a ligand for ALK, is secreted by the non-neural ectoderm during early neural crest migratory stages and internalized by neural crest cells in vivo. Similar to loss of LTK, loss of midkine reduces survival of the migratory neural crest. Moreover, we show by proximity ligation and co-immunoprecipitation assays that midkine binds to LTK. Taken together, these results suggest that LTK in neural crest cells interacts with midkine emanating from the non-neural ectoderm to promote cell survival, revealing a new signaling pathway that is essential for neural crest development. ; © 2018 Published by The Company of Biologists Ltd. Received February 6, 2018; Accepted September 13, 2018; Published 18 September 2018. We thank Marcos Simões-Costa and Stephen Roberts for kindly sharing reagents, Narmada Thayapran for technical assistance, and Yuwei Li for valuable comments on the manuscript. The authors declare no competing or financial interests. Author contributions: Conceptualization: F.M.V., M.E.B.; Methodology: F.M.V.; Formal analysis: F.M.V.; Investigation: F.M.V.; Data curation: F.M.V.; Writing - original draft: F.M.V., M.E.B.; Writing - review & editing: F.M.V., M.E.B.; Visualization: F.M.V.; Supervision: M.E.B.; Funding acquisition: F.M.V., M.E.B. This work was supported by a National Institutes of Health grant (R01DE024157 to M.E.B.) and postdoctoral fellowships from the Brazilian National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento CientÃfico e ...
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Leukocyte Receptor Tyrosine Kinase interacts with secreted midkine to promote survival of migrating neural crest cells
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Autor/in / Beteiligte Person: | Vieceli, Felipe Monteleone ; Bronner, Marianne E. |
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Zeitschrift: | Development, 145(20), Art. No. dev164046, (2018-10-24, 2018 |
Veröffentlichung: | Company of Biologists, 2018 |
Medientyp: | academicJournal |
DOI: | 10.1242/dev.164046 |
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