Stable knockdown of Sprouty4 in MCF10.DCIS cells promotes invasion.
2021
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Zugriff:
(A) MCF10.DCIS cells were infected with lentivirus containing either vector control or Sprouty4-targeting shRNA constructs. Membranes were immunoblotted for Sprouty4, Sprouty1, Sprouty2, and β-tubulin. (B) pLKO.1 DCIS control, pLKO.1 DCIS Sprouty4 knockdown, and MCF10.CA1d cells were then serum-starved overnight and allowed to invade though 1.5 mg/mL of matrix for 24 hours via Boyden chamber assay. Representative images of cellular invasion (scale bar = 500 μm). (C) Invasion was quantified and plotted using GraphPad Prism. A Kruskal-Wallis test employing Dunn’s test of multiple comparisons showed significant differences in invasion when comparing Sprouty4 knockdown or MCF10.CA1d cell lines to control (α = 0.01, 99% CI; ** p < 0.01, *** p < 0.001). Whiskers represent the minimum and maximum values and bars represent the median. Data were collected from three independent experiments.
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Stable knockdown of Sprouty4 in MCF10.DCIS cells promotes invasion.
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Autor/in / Beteiligte Person: | Ethan J. Brock (10892372) ; Ryan M. Jackson (10892375) ; Julie L. Boerner (8717202) ; Quanwen Li (113426) ; Meredith A. Tennis (10892378) ; Bonnie F. Sloane (10892381) ; Raymond R. Mattingly (113438) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0252314.g011 |
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