Einzeltreffer — DigiBib

Background: The transition from androgen-dependent to androgen-independent prostate cancer is not fully understood but appears to involve multiple genetic changes. We have identified a gene, GC79, that is more highly expressed in androgen-dependent LNCaP-FGC human prostate cancer cells than in androgen-independent LNCaP-LNO human prostate cancer cells. Physiologic levels (0.1 n M ) of androgens repress expression of GC79 messenger RNA (mRNA) in LNCaP-FGC cells. To determine the role of GC79, we cloned its complementary DNA (cDNA) and functionally characterized its product. Methods: The differentially expressed GC79 gene was cloned from human prostate cDNA libraries, sequenced, and transfected into mammalian cells to study its function. Expression of GC79 was analyzed in various adult and fetal human tissues and in prostate glands of castrated rats. The association of GC79 expression and apoptosis was investigated in COS-1 and LNCaP cells transfected with GC79 cDNA. All statistical tests are two-sided. Results: Sequence analysis indicates that GC79 encodes a large, complex, multitype zinc-finger protein, containing nine C 2 H 2 -type zinc-finger domains, a cysteine-rich region, and a GATA C 4 -type zinc-finger domain. Castration-induced androgen withdrawal increased the expression of GC79 mRNA in the regressing rat ventral prostate, suggesting that the expression of GC79 mRNA is associated with the process of apoptotic cell death in the rat ventral prostate. Transfection and induction of GC79 cDNA in both COS-1 and LNCaP prostate cancer cells led to an apoptotic index that was eightfold higher ( P <.001, two-sided Student's t test) than that observed in uninduced transfected cells. Conclusions: We have cloned an androgen-repressible gene, GC79, that is potentially involved in apoptosis. This finding may have implications for the development of androgen-independent prostate cancer and, ultimately, for the treatment of prostate cancer.

Titel:	Characterization of a Zinc-Finger Protein and Its Association With Apoptosis in Prostate Cancer Cells
Autor/in / Beteiligte Person:	Chang, Glenn T. G. ; Steenbeek, Martine ; Schippers, Esther ; Blok, Leen J. ; van Weerden, Wytske M. ; van Alewijk, Dirk C. J. G. ; Eussen, Bert H. J. ; van Steenbrugge, Gert J. ; Brinkmann, Albert O.
Link:	http://jnci.oxfordjournals.org/cgi/content/short/92/17/1414 https://doi.org/10.1093/jnci/92.17.1414
Veröffentlichung:	Oxford University Press, 2000
Medientyp:	academicJournal
DOI:	10.1093/jnci/92.17.1414
Schlagwort:	REPORTS
Sonstiges:	Nachgewiesen in: BASE Sprachen: English Collection: HighWire Press (Stanford University) Document Type: text File Description: text/html Language: English Rights: Copyright (C) 2000, National Cancer Institute

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Characterization of a Zinc-Finger Protein and Its Association With Apoptosis in Prostate Cancer Cells