Harnessing MalE for the study of antigen/antibody recognitions
In: ISSN: 0923-2508, 2002
academicJournal
Zugriff:
International audience ; The construction of hybrids between the variable fragment (Fv) of antibodies and protein MalE of Escherichia coli at the genetic level makes possible their preparation in a functional state, independently of any interaction with the antigen. We used such hybrids and a mutagenesis approach to study the recognition between antibody D1.3 and its antigen lysozyme, and its maturation. We subsequently transformed D1.3 into a reagentless fluorescent biosensor by knowledge-based design.
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Harnessing MalE for the study of antigen/antibody recognitions
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| Autor/in / Beteiligte Person: | Bedouelle, Hugues ; Renard, Martial ; Belkadi, Laurent ; England, Patrick ; Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry ; Institut Pasteur Paris ; Centre National de la Recherche Scientifique (CNRS) ; This research was funded by grants from the Ministry of National Education, Research and Technology (programme for fundamental research in microbiology, infectious and parasitic diseases) and from the Ministry of Defense (contract 99 34 043/DSP/STTC). |
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| Zeitschrift: | ISSN: 0923-2508, 2002 |
| Veröffentlichung: | HAL CCSD ; Elsevier, 2002 |
| Medientyp: | academicJournal |
| DOI: | 10.1016/s0923-2508(02)01337-2 |
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