Ο ρόλος της χημοκίνης CXCL8 (IL-8) και των υποδοχέων της CXCR1 και CXCR2 στην παθογένεια της στεφανιαίας νόσου ; Role of chemokine CXCL8 (IL-8) and its receptors CXCR1 and CXCR2 in coronary artery disease pathology

Atherosclerosis is a complicated multivariate disease of medium and large arteries, which leads to progressive accumulation of smooth muscle cells, fat and connective tissue in the vascular intima and is also connected with a lot of environmental risk factors interacting with the individuals genetic background. Growing evidence is obtained on the inflammatory nature of atherosclerosis. Several proinflammatory factors, chemoattractant cytokines (chemokines), binding molecules play an important role in orchestrating the overall procedure. In modern bibliography there is an increasing interest on the chemokines role in the atherosclerosis, because of their involvement in important aspects of atherogenesis, such as recruitment of inflammatory cells in vascular wall and proliferation of smooth muscle cells in atherosclerotic plaques. Interleukin 8 (IL-8), the standard chemokine of CXC subgroup appears to be involved in vascular pathology. Based on literature data, specific polymorphisms of the chemokine CXCL8 (IL-8) genes and its receptors are involved in the clinical manifestation of other diseases such as bronchial asthma, multiple sclerosis and RSV infection. In this context two of the five known polymorphisms were examined, the -251 A / T and 781 C / T of the promoter of the IL-8 gene and their frequency in relation to the existence or absence of coronary disease. For this purpose blood samples were collected from 300 CAD patients and 189 healthy controls. All the survey subjects were selected after undergoing Percutaneous Coronary Intervention (PCI). The DNA samples were divided in homozygous and heterozygous as for the studied polymorphism. Additionally there was also confirmation of the existence and position of the polymorphism -251 A/T by Sequencing. In the process of genotypic-phenotypic correlation 241 patients and 157 controls were included. In the same population was also recorded the clinical establishment of coronary heart disease (stable-unstable angina, acute myocardial infarction). This way it ...