The Reevaluation of Thrombin Time Using a Clot Waveform Analysis
In: Journal of Clinical Medicine, Vol 10, Iss 4840, p 4840 (2021, Jg. 10 (2021), Heft 4840, p 4840
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Zugriff:
Object: Although thrombin burst has attracted attention as a physiological coagulation mechanism, clinical evidence from a routine assay for it is scarce. This mechanism was therefore evaluated by a clot waveform analysis (CWA) to assess the thrombin time (TT). Material and Methods: The TT with a low concentration of thrombin was evaluated using a CWA. We evaluated the CWA-TT of plasma deficient in various clotting factors, calibration plasma, platelet-poor plasma (PPP), and platelet-rich plasma (PRP) obtained from healthy volunteers, patients with thrombocytopenia, and patients with malignant disease. Results: Although the TT-CWA of calibration plasma was able to be evaluated with 0.01 IU/mL of thrombin, that of FVIII-deficient plasma could not be evaluated. The peak time of CWA-TT was significantly longer, and the peak height significantly lower, in various deficient plasma, especially in FVIII-deficient plasma compared to calibration plasma. The second peak of the first derivative (1st DP-2) was detected in PPP from healthy volunteers, and was shorter and higher in PRP than in PPP. The 1st DP-2 was not detected in PPP from patients with thrombocytopenia, and the 1st DP-2 in PRP was significantly lower in patients with thrombocytopenia and significantly higher in patients with malignant disease than in healthy volunteers. Conclusion: The CWA-TT became abnormal in plasma deficient in various clotting factors, and was significantly affected by platelets, suggesting that the CWA-TT may be a useful test for hemostatic abnormalities.
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The Reevaluation of Thrombin Time Using a Clot Waveform Analysis
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Autor/in / Beteiligte Person: | Wada, Hideo ; Ichikawa, Yuhuko ; Ezaki, Minoru ; Matsumoto, Takeshi ; Yamashita, Yoshiki ; Shiraki, Katsuya ; Shimaoka, Motomu ; Shimpo, Hideto |
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Zeitschrift: | Journal of Clinical Medicine, Vol 10, Iss 4840, p 4840 (2021, Jg. 10 (2021), Heft 4840, p 4840 |
Veröffentlichung: | MDPI AG, 2021 |
Medientyp: | academicJournal |
ISSN: | 2077-0383 (print) |
DOI: | 10.3390/jcm10214840 |
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