In vitro GST-CBU1241 activity assays for MDH, LDH or ME function.
2021
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Zugriff:
(A) MDH activity was exhibited by 2 μg GST-CBU1241 and 0.013 units commercial pig heart mitochondria MDH, whereas 2 μg GST alone exhibited no activity in the MDH standard assay containing 2 mM OAA and 0.5 mM NADH in PBS pH 7.4. (B) LDH activity was not observed for 2 μg GST-CBU1241 or 2 μg GST in the LDH standard assay containing 2 mM pyruvate and 0.5 mM NADH in PBS pH 7.4. Conversely, 0.1 unit of commercial LDH enzyme had measurable activity. (C) Enzyme activity relative to pH 7.4 over a range of pHs, measured using MDH standard assay containing 1 μg GST-CBU1241. Activity was measured using increase or decrease of light absorbance at 340 nm, correlating to NADH oxidation and NAD + reduction respectively during enzyme activity. Error bars represent standard deviation from the mean from at least three independent experiments.
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In vitro GST-CBU1241 activity assays for MDH, LDH or ME function.
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Autor/in / Beteiligte Person: | Janine Hofmann (11273418) ; Mebratu A. Bitew (11273421) ; Miku Kuba (11273424) ; David P. De Souza (2793088) ; Hayley J. Newton (8692020) ; Fiona M. Sansom (8893082) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0255925.g003 |
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