Figure 1—figure supplement 4. Localization of EFHC-1 within cilia is largely static and independent of typical intraflagellar transport (IFT) mechanisms within cilia, the dendritic/ciliary transport protein, UNC-101, and the ciliary transcription factor, DAF-19. ; When distal regions of the CEP cilium in the EFHC-1::GFP overexpression strain (A) and middle regions of the OLQ cilium in the efhc-1::gfp CRISPR-knock-in strain (B) are photobleached, the GFP signal does not fully recover within 30 s. Pre-bleach ratios are normalized to 1. Scale bar, 3 μm. (C) Kymographs of IFT-20::GFP in ADL cilia (left), EFHC-1::GFP in CEP cilia (middle) and EFHC-1::GFP in OLQ cilia (right). PS, proximal segment. Scale bar, 3 μm (D) ODR-10 predominantly localizes to AWB olfactory cilia in wild-type, but it diffuses to dendrites in unc-101 (m1) mutant animals. Ciliary localizations of EFHC-1::GFP in OLQ and CEP neurons (arrows and arrowheads, respectively) are not disrupted in unc-101 mutants. Scale bar, 4 μm. (E) While CEP ciliary structure is disrupted in the absence of the ciliary DAF-19 transcription factor, EFHC-1::GFP is still expressed. Scale bar, 10 μm.

Figure 1—figure supplement 4. Localization of EFHC-1 within cilia is largely static and independent of typical intraflagellar transport (IFT) mechanisms within cilia, the dendritic/ciliary transport protein, UNC-101, and the ciliary transcription factor, DAF-19. ; When distal regions of the CEP cilium in the EFHC-1::GFP overexpression strain (A) and middle regions of the OLQ cilium in the efhc-1::gfp CRISPR-knock-in strain (B) are photobleached, the GFP signal does not fully recover within 30 s. Pre-bleach ratios are normalized to 1. Scale bar, 3 μm. (C) Kymographs of IFT-20::GFP in ADL cilia (left), EFHC-1::GFP in CEP cilia (middle) and EFHC-1::GFP in OLQ cilia (right). PS, proximal segment. Scale bar, 3 μm (D) ODR-10 predominantly localizes to AWB olfactory cilia in wild-type, but it diffuses to dendrites in unc-101 (m1) mutant animals. Ciliary localizations of EFHC-1::GFP in OLQ and CEP neurons (arrows and arrowheads, respectively) are not disrupted in unc-101 mutants. Scale bar, 4 μm. (E) While CEP ciliary structure is disrupted in the absence of the ciliary DAF-19 transcription factor, EFHC-1::GFP is still expressed. Scale bar, 10 μm.