SUB1A-1 anchors a regulatory cascade for epigenetic and transcriptional controls of submergence tolerance in rice
In: PNAS Nexus, 2023
academicJournal
Zugriff:
Most rice (Oryza sativa) cultivars cannot survive under prolonged submergence. However, some O. sativa ssp. indica cultivars, such as FR13A, are highly tolerant owing to the SUBMERGENCE 1A-1 (SUB1A-1) allele, which encodes a Group VII ethylene-responsive factor (ERFVII) protein; other submergence-intolerant cultivars contain a SUB1A-2 allele. The two alleles differ only by a single substitution at the 186(th) amino acid position from serine in SUB1A-1 to proline in SUB1A-2 resulting in only SUB1A-1 being able to be phosphorylated. Two other ERFVIIs, ERF66 and ERF67, function downstream of SUB1A-1 to form a regulatory cascade in response to submergence stress. Here, we show that SUB1A-1, but not SUB1A-2, interacts with ADA2b of the ADA2b-GCN5 acetyltransferase complex, in which GCN5 functions as a histone acetyltransferase. Phosphorylation of SUB1A-1 at serine 186 enhances the interaction of SUB1A-1 with ADA2b. ADA2b and GCN5 expression was induced under submergence, suggesting that these two genes might play roles in response to submergence stress. In transient assays, binding of SUB1A-1 to the ERF67 promoter and ERF67 transcription were highly induced when SUB1A-1 was expressed together with the ADA2b-GCN5 acetyltransferase complex. Taken together, these results suggest that phospho-SUB1A-1 recruits the ADA2-GCN5 acetyltransferase complex to modify the chromatin structure of the ERF66/ERF67 promoter regions and activate gene expression, which in turn enhances rice submergence tolerance.
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SUB1A-1 anchors a regulatory cascade for epigenetic and transcriptional controls of submergence tolerance in rice
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Autor/in / Beteiligte Person: | Lin, Chih-Cheng ; Lee, Wan-Jia ; Zeng, Cyong-Yu ; Chou, Mei-Yi ; Lin, Ting-Jhen ; Lin, Choun-Sea ; Ho, Meng-Chiao ; Shih, Ming-Che |
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Zeitschrift: | PNAS Nexus, 2023 |
Veröffentlichung: | Oxford University Press, 2023 |
Medientyp: | academicJournal |
DOI: | 10.1093/pnasnexus/pgad229 |
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